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Letters to Nature

Nature 240, 404-406 (15 December 1972) | doi:10.1038/240404a0

Holography without Fringes in the Electron Microscope

R. W. GERCHBERG

  1. Cavendish Laboratory, Cambridge
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I REPORT here the successful solution of the phase problem for a negatively stained catalase crystal using diffraction and image plane pictures produced in an electron microscope. The results show the complete wave function, phase as well as amplitude maps, in-the microscope image plane. The computational method employed in achieving the solution has been described by Gerchberg and Saxton1 who demonstrated the technique on general mathematical functions; the method was later successfully applied to mathematical models of magnetic thin films2. I have now applied the method to a real biological crystal in the electron microscope.