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White-light Three Dimensional Microscopy using Multiple-image Storing and Decoding

Abstract

THERE is a need in microscopy for high quality imaging of transparent specimens which have a large axial depth compared with the depth of focus. Because of the necessarily limited axial depth of focus of conventional microscopes, when a biologist needs detailed information throughout the thickness of a specimen, without compromising lateral resolution, he has to take a series of successive photographs on separate plates (or film frames).

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BURKE, J., INDEBETOUW, G., NOMARSKI, G. et al. White-light Three Dimensional Microscopy using Multiple-image Storing and Decoding. Nature 231, 303–306 (1971). https://doi.org/10.1038/231303a0

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  • DOI: https://doi.org/10.1038/231303a0

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