Nature 224, 1164 - 1166 (20 December 1969); doi:10.1038/2241164a0

Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase

PAULA DE LUCIA & JOHN CAIRNS

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

By testing indiscriminately several thousand colonies of mutagenized E. coli, a mutant has been isolated that on extraction proves to have less than I per cent of the normal level of DNA polymerase. The mutant multiplies normally but has acquired an increased sensitivity to ultraviolet light.

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