Nature 219, 1021 - 1025 (07 September 1968); doi:10.1038/2191021a0

Mechanism of Lymphocyte Transformation induced by Phytohaemagglutinin

M. J. SIMONS, R. FOWLER & M. G. FITZGERALD

Immunology Section, Surgical Research Department, Royal Children's Hospital Research Foundation
Cytogenetics Laboratory, Department of Pathology, Royal Children's Hospital, Melbourne, Australia

The role of phytohaemagglutinin (PHA) determinants and lymphocyte surface reactive sites has been investigated in the lymphocyte transformation phenomenon. Results suggest that the mitogenic moiety of the PHA molecule is closely related to or identical with the antigenic determinant sites, and that some of these PHA antigenic sites are similar to those found amongst the antigenic configuration on the surface of lymphocytes.

1. Nowell, P. C. , Cancer Res., 20, 462 (1960). | PubMed | ISI | ChemPort |
2. Robbins, J. H. , Science, 146, 1648 (1964). | PubMed | ISI | ChemPort |
3. Bach, F. , and Hirschhorn, K. , Seminars in Haematol., 2 (1), 68 (1965).
4. The Lymphocyte in Immunology and Haemopoiesis (edit. by Yoffey, J. M.) (Arnold, 1967).
5. Coulson, A. S. , and Chalmers, D. G. , Nature, 209, 378 (1966). | Article | PubMed | ISI | ChemPort |
6. Yam, L. T. , Castoldi, G. L. , and Mitus, W. J. , J. Lab. Clin. Med., 70, 699 (1967). | PubMed | ISI | ChemPort |
7. Moorhead, J. F. , Connolly, J. J. , and McFarland, W. , J. Immunol., 99, 413 (1967). | PubMed | ISI | ChemPort |
8. Wilson, D. B. , J. Exp. Zool., 162, 161 (1966). | Article | ISI |
9. Simons, M. J. , and Fitzgerald, M. G. , Proc. Austral. Soc. Med. Res., 2, 121 (1967).
10. Gräsbeck, R. , Nordman, C. T. , and de la Chapelle, A. , Lancet, i, 385 (1963).
11. Caron, G. A. , and Sarkany, I. , Nature, 210, 314 (1966). | Article | PubMed | ISI | ChemPort |
12. Marshall, W. H. , and Mellman, S. , Clin. Exp. Immunol., 1, 189 (1966). | PubMed | ChemPort |
13. Pearmain, G. , Lycette, R. R. , and Fitzgerald, P. H. , Lancet, i, 637 (1963).
14. Elves, M. W. , and Israels, M. G. C. , Brit. J. Haematol., 9, 406 (1963). | ISI | ChemPort |
15. Forsdyke, D. R. , in The Biological Effects of Phytohaemagglutinin (edit. by Elves, M. W.), 115 (Robert Jones and Agnes Hunt Orthopaedic Hospital Management Committee, Oswestry, 1966).
16. Abaza, H. M. , and Woodruff, M. F. A. , Revue fr. Etud. Clin. Biol., 11, 821 (1966). | ISI |
17. Byrd, W. J. , Finley, W. H. , Finley, S. C. , and McLure, S. , Lancet, i, 420 (1964).
18. Spitz, M. , Nature, 202, 902 (1964). | PubMed | ISI | ChemPort |
19. Marshall, W. H. , and Norins, L. C. , Austral. J. Exp. Biol. Med., Sci., 43, 213 (1965). | ISI | ChemPort |
20. Ling, N. R. , Knight, S. , Hardy, D. , Stanworth, D. R. , and Holt, P. J. L. , in Anti-lymphocyte Serum, Ciba Foundation Study Group No. 29, 41 (1967).
21. Woodruff, M. F. A. , James, K. , Anderson, N. F. , and Reid, B. L. , in Anti-lymphocyte Serum, Ciba Foundation Study Group No. 29, 57 (1967).
22. Simons, M. J. , Proc. Austral. Soc. Med. Res., 2, 121 (1967).
23. Greaves, M. F. , Roitt, I. M. , Zamir, R. , and Carnaghan, R. B. A. , Lancet, ii, 1317 (1967).
24. Sell, S. , and Gell, P. G. H. , J. Exp. Med., 122, 923 (1965). | Article | PubMed | ISI | ChemPort |
25. Adinolfi, M. , Gardner, B. , Gianelli, F. , and McGuire, M. , Experientia, 23, 271 (1967). | Article | PubMed | ISI | ChemPort |
26. Gell, P. G. H. , in Anti-lymphocyte Serum, Ciba Foundation Study Group, No. 29, 50 (1967).
27. Copperman, R. , and Morton, H. E. , Proc. Soc. Exp. Biol. and Med., 123, 790 (1966). | ISI | ChemPort |
28. Olson, G. B. , South, M. A. , and Good, R. A. , Nature, 214, 695 (1967). | Article | PubMed | ISI | ChemPort |