Nature 216, 65 - 66 (07 October 1967); doi:10.1038/216065a0

Haematopoietic Stem Cells: Evidence for Competing Proliferative Demands

SAMUEL HELLMAN & HELEN E. GRATE

Department of Radiology, Yale University School of Medicine, New Haven, Connecticut.

A HAEMATOPOIETIC stem cell must be able both to replicate itself and to differentiate into a formed blood element. The presence of a pool of multipotent, functionally important, common stem cells for the various haematopoietic formed elements has not as yet been established. There is evidence to suggest that there is a population of bone marrow cells which when transplanted into irradiated recipients is capable of differentiating into more than one mature cellular form^1–3. We are interested in whether this class of cells is functionally important, and specifically whether demand for one blood element limits the ability of the animal to produce other cell types. Such a relationship would suggest competitive demands on a common stem cell pool. There is some evidence to suggest that this may be the case. Harris et al. ⁴ have shown that after large acute haemorrhage the number of granulocyte precursors in the guinea-pig bone marrow is reduced. The present experiment demonstrates that an increased demand for red blood cell production reduces the ability of transplanted syngeneic bone marrow to make granulocytic progeny.

1. Barnes, D. W. H. , Ford, C. E. , Gray, S. M. , and Loutit, J. F. , in Progress in Nuclear Energy, Series 6, 2, 1–10 (edit. by Bugher, J. G., Coursaget, J., and Loutit, J. F.) (Pergamon Press, London, 1959).
2. Bryant, B. J. , and Cole, L. J. , in The Lymphocyte in Immunology and Haemopoiesis, 170 (Edward Arnold Ltd., 1966).
3. Wu, A. M. , Till, J. E. , Siminovitch, L. , and McCulloch, E. A. , J. Cell Physiol. (in the press).
4. Harris, P. F. , Harris, R. S. , and Kugler, J. H. , Brit. J. Haematol., 12, 419 (1966). | ISI | ChemPort |
5. Hellman, S. , and Grate, H. E. , Blood, 30, 103 (1967). | PubMed | ISI | ChemPort |
6. Craddock, jun., C. S. , Perry, S. , Ventske, L. E. , and Lawrence, J. S. , Blood, 15, 840 (1960). | PubMed | ISI |
7. Alpen, E. L. , and Cranmore, D. , in Kinetics of Cellular Proliferation, 290 (Grune and Stratton, New York, 1961).
8. Erslev, A. J. , Blood, 14, 386 (1959). | ISI |
9. Erslev, A. J. , Blood, 24, 331 (1964). | PubMed | ISI | ChemPort |
10. Fisher, R. A. , in Statistical Methods for Research Workers, 99 (Oliver and Boyd, London, 1958).
11. Liron, M. , and Feldman, M. , Israel J. Med. Sci., 1, 86 (1965). | ISI |
12. Bleiberg, I. , Liron, M. , and Feldman, M. , Transplantation, 3, 706 (1965). | PubMed | ChemPort |
13. Curry, J. L. , Trentin, J. J. , and Wolf, N. , J. Exp. Med., 125, 703 (1967). | Article | PubMed | ISI | ChemPort |
14. Bleiberg, I. , Liron, M. , and Feldman, M. , Blood, 29, 469 (1967). | PubMed | ISI | ChemPort |