Abstract
THE development of liquid chromatographic techniques has been restricted because of the non-existence of sensitive detection systems. Apart from the tedious manual analysis of evaporation and determination by weight or titration the only available continuous method has been ultra-violet absorption. In this process, the sample, for example amino-acid, peptide or protein, is developed by an external reagent like ninhydrin to give strong absorption in the ultra-violet. This procedure is carried out at a single wave-length and is restricted to those samples with the required characteristic.
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References
U.K. Patent, No. 998, 107.
Chem. and Indust., 746 (May 1964).
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MAKIN, B., SPEAKMAN, F. Advances in Liquid Chromatography. Nature 208, 1087–1088 (1965). https://doi.org/10.1038/2081087a0
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DOI: https://doi.org/10.1038/2081087a0
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