Abstract
EVEN though the presence of blood in tissue homogenates is quite evident, its implication in the interpretation of results is generally ignored. In work on rat liver tissue, this attitude results from an under-estimation of the blood content by earlier investigators1–4. In order to gauge to what extent blood ‘contamination’ must be taken into account, a very accurate determination of blood content of homogenates is essential. A simple and accurate method, free from the errors inherent in procedures used so far, is described here. It consists in (1) complete extraction of haemoglobin from liver with sodium deoxycholate (DOC) followed by a specific spectrophotometric determination of haemoglobin; (2) evaluation of blood content in homogenates by comparing it with the blood from the same animal.
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CASTAGNA, M. Blood Content of Rat Liver Homogenates. Nature 205, 905–907 (1965). https://doi.org/10.1038/205905a0
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DOI: https://doi.org/10.1038/205905a0
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