Nature 204, 385 (24 October 1964); doi:10.1038/204385a0

Assay of Gonadotrophins by the Deciduoma Method

NADA KOVAI

United Birmingham Hospitals, Department of Clinical Endocrinology, Birmingham and Midland Hospital for Women, Showell Green Lane, Birmingham, 11.

IN this laboratory a method has been developed for measuring the luteotrophic activity of prolactin by the formation of deciduomata in a damaged uterine horn in the hypophysectomized adult mouse¹. The decidual response was quantal and the percentage of positive responses was transformed into probits. The method was designed as a 4 or 6 point parallel line assay and results were expressed in terms of the 2nd International Standard Prolactin (ovine prolactin, Armour Lab., Lot No. D 14083–2B, potency 22 I.U./mg).

1. Kovai, Nada , J. Endocrin., 28, 45 (1963).
2. Hartree, Anne S. , Butt, W. R. , and Kirkham, K. , J. Endocrin., 29, 61 (1964). | ISI | ChemPort |