Nature 202, 187 - 188 (11 April 1964); doi:10.1038/202187a0

Application of Particle Electrophoresis to the Detection of Antibody bound to Cells of Streptococcus pyogenes

M. J. HILL, A. M. JAMES & W. R. MAXTED

Department of Chemistry, Chelsea College of Science and Technology, Manresa Road, London, S.W.3.
Streptococcal Reference Laboratory, Central Public Health Laboratory, Colindale, London, N.W.9.

ALTHOUGH it has rarely been used for this purpose the microelectrophoretic technique¹ is well suited for the detection of antibody adsorbed to bacterial cells. The antigen–antibody reactions between a single strain of Streptococcus pyogenes type 6 matt variant (N.C.T.C. 8302) and antisera for its type-specific M and T antigens, and for the group specific C antigen, have been investigated in this way. The type-specific sera were fully absorbed to remove heterologous cross-reactions. The group-specific sera were unabsorbed but showed no cross-reactions with other groups.

1. Gittens, G. J. , and James, A. M. , Anal. Biochem., 1, 478 (1960). | Article | PubMed | ISI | ChemPort |
2. Hill, M. J. , James, A. M. , and Maxted, W. R. , Biochim. Biophys. Acta, 66, 264 (1963). | Article | PubMed | ISI | ChemPort |
3. Hill, M. J. , James, A. M. , and Maxted, W. R. , Biochim, Biophys. Acta, 75, 402 (1963). | Article | ISI | ChemPort |