Nature 196, 901 (01 December 1962); doi:10.1038/196901a0

Change in A³ Hæmoglobin due to -Chain

JEAN ROSA & DOMINIQUE LABIE

Laboratoire de Recherches de Biochimie Médicale, Hôpital des Enfants Malades, Paris.

SEVERAL authors^1–3 have shown that the minor anodic fraction of hæmoglobin obtained by electrophoresis at pH 8.6 is part of the hæmoglobin modified during the ageing of the red cells. Proof is submitted here that the modification of the electrical charge of hæmoglobin A³ (Hb^A₃) is due only to the modification of the -chain. This is proved by comparison of the electrophoretic migration of the hybrids obtained between canine hæmoglobin Hb^CAN and Hb^A and between Hb^CAN and Hb^A₃.

1. Rosa, J. , Dreyfus, J. C. , and Schapira, G. , Nature, 188, 753 (1960). | PubMed | ISI | ChemPort |
2. Rosa, J. , Schapira, G. , and Dreyfus, J. C. , Bull. Soc. Chim. Biol., 43, 555 (1961). | PubMed | ISI | ChemPort |
3. Meyering, C. A. , Israels, A. L. M. , Sebens, T. , and Huisman, T. H. J. , Clin. Chim. Acta, 5, 208 (1960). | Article | ISI |
4. Itano, H. A. , and Robinson, E. , Nature, 184, 1468 (1959). | PubMed | ISI | ChemPort |
5. Labie, D. , Rosa, J. , Dreyfus, J. C. , and Schapira, G. , Nature, 194, 384 (1962). | Article | PubMed | ISI | ChemPort |
6. Clegg, M. D. , and Schroeder, W. A. , J. Amer. Chem. Soc., 81, 6065 (1959). | Article | ISI | ChemPort |
7. Kunkel, H. G. , and Wallenius, G. , Science, 122, 288 (1955). | PubMed | ISI | ChemPort |
8. Smithies, O. , Biochem. J., 61, 629 (1955). | PubMed | ISI | ChemPort |
9. Allen, D. W. , and Jandl, J. H. , J. Clin. Invest., 40, 454 (1961). | PubMed | ISI | ChemPort |