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Nature 195, 735 - 736 (18 August 1962); doi:10.1038/195735a0

A Sensitive Polarographic Test for the Detection of Protein Impurities in Viral Nucleic Acid

G. RUTTKAY-NEDECKÝ & V. ScaronPÁNIK

Institute of Virology, Czechoslovak Academy of Sciences, Bratislava, Czechoslovakia.

RECENTLY it was shown that the polarographic method is suitable for the determination of small amounts (0.2 per cent and more) of non-virus protein in tobacco mosaic virus (TMV) preparations1. In further experiments we have found this method equally suitable for the detection of traces of protein contaminants in the infective ribonucleic acid (RNA) preparations, isolated from TMV by the phenol method according to Gierer and Schramm2.

  1. Ruttkay-Nedecký, G. , Biochem. Biophys. Acta, 26, 455 (1957). Coll. Czechoslov. Chem. Comm. (in the press). | PubMed |
  2. Gierer, A. , and Schramm, G. , Z. Naturforsch., 11, b, 138 (1956). | ISI |
  3. Berg, H. , Biochem. Z., 329, 274 (1957). | PubMed | ISI | ChemPort |
  4. Brdic caronka, R. , Coll. Czechoslov. Chem. Comm., 5, 112 (1933).
  5. Franklin, R. E. , Klug, A. , and Holmes, K. C. , Ciba Found. Symp. Nature of Viruses (Churchill, Ltd., London, 1957).
  6. Anderer, F. A. , Z. Naturforsch., 14, b, 24, 642 (1959). | ISI |
  7. Gierer, A. , Z. Naturforsch., 13, b, 477, 485 (1958). | ISI |



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