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Preservation of Bull and Human Spermatozoa by Freezing in Liquid Nitrogen Vapour

Abstract

MY work on factors involved in low-temperature preservation has favoured the design of a simple and efficient freezing method for the preservation of spermatozoa of the bull and the human. I have used this method successfully for more than three years and find it as good as, or superior to, present methods, including those requiring expensive automatic freezing equipment. It uses liquid nitrogen and is an improvement over ‘dry ice’ methods.

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References

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SHERMAN, J. Preservation of Bull and Human Spermatozoa by Freezing in Liquid Nitrogen Vapour. Nature 194, 1291–1292 (1962). https://doi.org/10.1038/1941291a0

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