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| Nature 193, 1289 - 1290 (31 March 1962); doi:10.1038/1931289a0 |
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Preparation of Tritium-labelled Paromomycin ('Humatin') by Fermentation in a Medium containing Tritiated Water
ROBERT E. OBER, SALVATORE A. FUSARI, GEORGE L. COFFEY, G. WAYNE GWYNN & ANTHONY J. GLAZKO
Research Division, Parke, Davis and Co., Ann Arbor and Detroit, Michigan.
THE antibiotic paromomycin is a basic, water-soluble compound with a general structure similar to that of neomycin and kanamycin1. Failure to obtain radio-chemically pure paromomycin following exposure to tritium gas by the Wilzbach technique2 prompted us to try biosynthesis. While biosynthetic incorporation of hydrogen isotope from water into organic compounds has been reported frequently in the literature3–5, this technique has not been used widely for preparation of tritium-labelled compounds. While the work reported here was in progress, Dutton et al. 6 reported the isolation of tritium-labelled fatty acids from soybeans grown in a nutrient solution containing tritiated water.
- Haskell, T. H. , French, J. C. , and Bartz, Q. R. , J. Amer. Chem. Soc., 81, 3482 (1959). | Article | ISI | ChemPort |
- Wilzbach, K. E. , J. Amer. Chem. Soc., 79, 1013 (1957). | Article | ISI | ChemPort |
- Schoenheimer, R. , and Rittenberg, D. , Physiol. Rev., 20, 218 (1940). | ChemPort |
- Chorney, W. , Scully, N. J. , Crespi, H. L. , and Katz, J. J. , Biochim. Biophys. Acta, 37, 280 (1960). | Article | PubMed | ISI | ChemPort |
- Moses, V. , and Calvin, M. , Biochim. Biophys. Acta, 33, 297 (1959). | Article | PubMed | ISI | ChemPort |
- Dutton, H. J. , Jones, E. P. , Scholfield, C. R. , Chorney, W. , and Scully, N. J. , J. Lipid Res., 2, 63 (1961). | ISI | ChemPort |
- Coffey, G. L. , Anderson, L. E. , Fisher, M. W. , Galbraith, M. M. , Hillegas, A. B. , Kohberger, D. L. , Thompson, P. E. , Weston, K. S. , and Ehrlich, J. , Antibiotics and Chemotherapy, 9, 730 (1959). | ChemPort |
- Kelly, R. G. , Peets, E. A. , Gordon, S. , and Buyske, D. A. , Anal. Biochem., 2, 267 (1961). | Article | PubMed | ISI | ChemPort |
© 1962 Nature Publishing Group
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