Nature 189, 775 - 776 (04 March 1961); doi:10.1038/189775a0

Chloramphenicol, a Simultaneous Carbon and Nitrogen Source for a Streptomyes sp. from Egyptian Soil

Y. ABD-EL-MALEK, M. MONIB & A. HAZEM

Department of Agricultural Bacteriology, Faculty of Agriculture, University of Cairo.

IN work concerning the decomposition of antibiotics in soil and micro-organisms capable of this process, a chloramphenicol solution (250 gm. chloramphenicol/gm. soil) was percolated through 25-gm. sieved fresh garden soil, PH 7.5, in an apparatus similar to that described by Lees and Quastel¹. The chloramphenicol was applied in three doses; the first dissolved in 50 ml. sterile distilled water and the others in 5 ml. added to the percolate in the reservoir. The antibiotic was bioassayed daily against Bacillus subtilis. The first dose disappeared after 11 days and the second and third after 3 days.

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