Nature 187, 1127 - 1128 (24 September 1960); doi:10.1038/1871127a0

Chromatography of Blood Thrombokinase on 'DEAE'-Cellulose

J. H. MILSTONE

Department of Pathology, Yale University School of Medicine, New Haven, Connecticut.

THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations¹. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day².

1. Milstone, J. H. , Proc. Soc. Exp. Biol. and Med., 103, 361 (1960). | ISI | ChemPort |
2. Sober, H. A. , Gutter, F. J. , Wyckoff, M. M. , and Peterson, E. A. , J. Amer. Chem. Soc., 78, 756 (1956). | Article | ISI | ChemPort |
3. Milstone, J. H. , J. Gen. Physiol., 42, 665 (1959). | Article | PubMed | ISI | ChemPort |
4. Lowry, O. H. , Rosebrough, N. J. , Farr, A. L. , and Randall, R. J. , J. Biol. Chem., 193, 265 (1951). | PubMed | ISI | ChemPort |
5. Mellanby, J. , and Pratt, C. L. G. , Proc. Roy. Soc., B, 128, 201 (1939).
6. Sherry, S. , and Troll, W. , J. Biol. Chem., 208, 95 (1954). | PubMed | ISI | ChemPort |
7. Milstone, J. H. , J. Gen. Physiol., 38, 757 (1955). | Article | PubMed | ISI | ChemPort |
8. Macfarlane, R. G. , J. Physiol., 106, 104 (1947). | ISI | ChemPort |
9. Arscott, P. M. , Koppel, J. L. , and Olwin, J. H. , Nature, 183, 753 (1959). | PubMed | ISI | ChemPort |