  | |||||||||||||||||||
|
|||||||||||||||||||
|
|||||||||||||||||||
|
|||||||||||||||||||
|
|||||||||||||||||||
| Journal Home | |
| Current Issue | |
| AOP | |
| Archive | |
| |
| |
THIS ARTICLE  | |
| |
| Download PDF | |
| References | |
| |
| |
| |
| Export citation | |
| Export references | |
| |
| |
| |
| Send to a friend | |
| |
| |
| |
| More articles like this | |
| |
| |
| |
| Table of Contents | |
| < Previous | Next > | |
| |
 |
 |
| Nature 182, 813 - 814 (20 September 1958); doi:10.1038/182813a0 |
|
Dependence of Mitochondrial Swelling on Oxidizable Substrates
J. B. CHAPPELL & G. D. GREVILLE
Department of Biochemistry, University of Cambridge. Aug. 11.
THAT the swelling of isolated mitochondria induced by various agents fails to occur under anaerobic conditions1,2 suggested to us that swelling may be dependent on respiration. The probability of this is increased by the finding3,4 that malonate prevents the swelling induced by succinate of mitochondria suspended in mannitol or sucrose solutions. We have therefore studied the effects of addition of substrates and inhibitors of oxidative metabolism on the swelling of mitochondria under various conditions. Mitochondria from livers of male rats (180–280 gm. body-weight) were prepared, suspended and stored in 0.44 M sucrose at 0° C. 0.05 ml. of mitochondrial suspension (25 mgm.-equivalents; 0.1 mgm. nitrogen) was added to 2.95 ml. of medium containing 2-amino-2-hydroxymethylpropane-1 : 3-diol (tris) chloride buffer, pH 7.5, and sucrose (final concentrations 25 mM and 0.3 M respectively). The optical density at 520 m
. (D
520) was recorded for periods up to 1 hr., the temperature being thermostatically controlled at 20°. Decreases in D520were taken as a measure of swelling. Substrates were used in 1 mM concentration.
- Hunter, F. E. , Davis, J. , and Carlat, L. , Biochim. Biophys. Acta, 20, 237 (1956). | PubMed | ISI | ChemPort |
- Lehninger, A. L. , and Ray, B. L. , Biochim. Biophys. Acta, 26, 643 (1957). | Article | PubMed | ISI | ChemPort |
- Raaflaub, J. , Helv. Physiol. Pharmacol. Acta, 11, 142 (1953). | PubMed | ISI | ChemPort |
- Tapley, D. F. , J. Biol. Chem., 222, 325 (1956). | PubMed | ISI | ChemPort |
- Ernster, L. , Jalling, O. , Löw, H. , and Lindberg, O. , Exp. Cell Res., Supp. 3, 124 (1955). | ChemPort |
- Potter, V. R. , and Reif, A. E. , J. Biol. Chem., 194, 287 (1952). | PubMed | ISI | ChemPort |
- Johnson, D. , and Lardy, H. A. , Nature, 181, 701 (1958). | PubMed | ISI | ChemPort |
© 1958 Nature Publishing Group
Privacy Policy
