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| Nature 181, 1419 - 1420 (17 May 1958); doi:10.1038/1811419a0 |
|
Antibody Production by Single Cells
G. J. V. NOSSAL & JOSHUA LEDERBERG*
Walter and Eliza Hall Institute of Medical Research, Melbourne. March 25.
*Fulbright Visiting Professor of Bacteriology, University of Melbourne; from Department of Medical Genetics, University of Wisconsin, Madison.
FAGREUS1 and others2,3 have shown that certain tissues from pre-sensitized animals can form antibody in vitro. This communication describes a technique whereby antibody production by single cells isolated in microdroplets can be detected. The technique is based on specific immobilization of Salmonella serotypes by anti-flagellar antibody. It was observed that single cells from a rat, simultaneously stimulated with two antigens, formed detectable amounts of one or the other antibody.
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© 1958 Nature Publishing Group
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