Abstract
IN 1932, Dr. Kurt Stern published a German translation of my book “Enzymes”1, with numerous additions to the English text. On pp. 119–120, I described some graphical methods, stating that they were due to my friend Dr. Barnett Woolf. Michaelis's equation may be written , where x is the substrate concentration at any moment, v the velocity with which the substrate is being destroyed, V the velocity when the enzyme is saturated, and K the Michaelis constant. Woolf pointed out that linear graphs are obtained when v is plotted against vx −1, v −1 against x −1, or v −1 x against x, the first plot being most convenient unless inhibition is being studied. But competitive inhibition gives a pencil of lines through the point (0,V −1), while non-competitive inhibition gives a pencil through the point (− K −1, 0), when v −1 is plotted against x −1.
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References
Haldane, J. B. S., and Stern, K., “Allgemeine Chemie der Enzyme” (Steinkopf, Leipzig and Berlin, 1932).
Lineweaver, H., and Burk, D., J. Amer. Chem. Soc., 56, 658 (1934).
Hofstee, B. H. J., Enzymologia, 17, 273 (1956).
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HALDANE, J. Graphical Methods in Enzyme Chemistry. Nature 179, 832 (1957). https://doi.org/10.1038/179832b0
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DOI: https://doi.org/10.1038/179832b0
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