Nature 178, 492 - 493 (01 September 1956); doi:10.1038/178492b0

Detection of Xanthine Dehydrogenase Activity in Soluble Proteins of Rat Liver separated by Paper Electrophoresis

EMILIO MITIDIERI, OTTILIA R. AFFONSO, LUIZ P. RIBEIRO & GILBERTO G. VILLELA

Biochemical Laboratory, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.

IT has been shown by paper electrophoresis that xanthine dehydrogenase activity is bound to the globulin fractions of rat serum¹. Using differential centrifugation, it was demonstrated that xanthine oxidase and xanthine dehydrogenase activities are present in the supernatant fluid corresponding to the 'cell sap' containing the soluble proteins of the rat liver².

1. Mitidieri, E. , Ribeiro, L. P. , Affonso, O. R. , and Villela, G. G. , Biochim. Biophys. Acta, 17, 587 (1955). | Article | PubMed | ISI | ChemPort |
2. Villela, G. G. , Mitidieri, E. , and Affonso, O. R. , Nature, 175, 1087 (1955). | PubMed | ISI | ChemPort |
3. Adjutantis, G. , Nature, 173, 539 (1954). | PubMed | ISI | ChemPort |
4. Hogeboom, G. H. , Schneider, W. C. , and Pallade, G. E. , J. Biol. Chem., 172, 619 (1948). | ISI | ChemPort |
5. Roush, A. , and Norris, E. R. , Arch. Biochem., 29, 344 (1950). | PubMed | ISI | ChemPort |
6. Villela, G. G. , Rev. Brasil. Biol., 14, 455 (1954).
7. Svedberg, T. , and Pedersen, K. O. , "The Ultracentrifuge", 296 (Oxford, 1940).
8. Villela, G. G. , and Mitidieri, E. , Nature, 175, 208 (1955). | Article | PubMed | ISI | ChemPort |
9. Affonso, O. R. , Mitidieri, E. , Ribeiro, L. P. , and Villela, G. G. , Proc. Soc. Exp. Biol. Med., 90, 527 (1955). | ISI | ChemPort |