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| Nature 175, 478 (12 March 1955); doi:10.1038/175478a0 |
|
Use of Triethylammonium Buffers in Ion-Exchange Chromatography and Electrophoresis
JERKER PORATH
Institute of Biochemistry, University of Uppsala. Nov. 11.
IN preparative ion-exchange chromatography or electrophoresis of mixtures of low-molecular non-volatile compounds, it is essential to use buffer salts that are easily removed. Many methods have been employed or proposed for desalting. Of these, only evaporation of the buffer can, in our opinion, be considered as entirely satisfactory, since it introduces no other operation than the one required for the removal of the solvent. The number of volatile buffers in use so far is much limited, and those buffers do not meet all demands.
- Michl, H. , Mh. Chem., 82, 489 (1951). | ChemPort |
- Newton, G. G. F. , and Abraham, E. P. , Biochem. J., 58, 103 (1954). | PubMed | ISI | ChemPort |
- Hirs, C. W. H. , Moore, S. , and Stein, W. H. , J. Biol. Chem., 195, 669 (1952). | PubMed | ISI | ChemPort |
- Moore, S. , and Stein, W. H. , J. Biol. Chem., 176, 367 (1948). | ISI | ChemPort |
© 1955 Nature Publishing Group
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