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Cultivation of Rabbit Eggs and Cumuli for Phase-Contrast Microscopy

Nature volume 164, pages 1136–1137 (1949)Cite this article

Abstract

PHASE-CONTRAST microscopy has been used to study cultures of chick embryonic tissue1 and mouse spleen2, but not, hitherto, for observing cultures of mammalian eggs. The methods described by Pincus3 and Chang4 for cultivation of rabbit eggs are not designed for phase microscopy, and histological procedures are required to show the nuclei. Austin and Smiles5 demonstrated the value of the phase-contrast microscope in a study of rat eggs freshly recovered from the Fallopian tubes ; nuclei could be seen in the intact eggs, and much more clearly after they had been somewhat flattened between slide and coverslip.

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References

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Authors and Affiliations

  1. National Institute for Medical Research, Hampstead, N.W.3

    A. U. SMITH

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  1. A. U. SMITH
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SMITH, A. Cultivation of Rabbit Eggs and Cumuli for Phase-Contrast Microscopy. Nature 164, 1136–1137 (1949). https://doi.org/10.1038/1641136b0

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