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Nature 161, 316-317 (28 February 1948) | doi:10.1038/161316b0;
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Cytology of Staphylococci Before and After Treatment with Penicillin
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Abstract
As part of a general programme of research, we have investigated cytological variations of a staphylococcus strain (pyogenic Staphylococcus aureus isolated from a human boil), the bacteria having been cultivated either (1) on ordinary agar broth medium, or (2) on agar broth medium containing a sublethal concentration of penicillin (75 units per litre of medium for this bacterium) which delays growth without stopping it. A. NORMAl· STAPHYLOCOCCTTS. GENERAL BASOPHILY. B. NORMAL STAPHYLOCOCCUS. NORMAL SWELLING DURING THE LAG PHASE. C. THE SAME, AFTER RIBONUCLEASE TREATMENT: NUCLEI. D. STAPHYLOCOCCUS TREATED WITH PENICILLIN. DEMONSTRATION OF THE BASOPHILIC MASSES. E. STAPHYLOCOCOUS TREATED WITH PENICILLIN, AFTFR RIBONUCLEASE TREATMENT I NUCLEI. (ON THE EIGHT, EMPTY SHELL; CYTOLIZED CELL?) (DRAWN FROM PHOTOMICROGRAPHS) Staphylococci cultivated on agar broth medium without penicillin.
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