Abstract
IN daily observations on the structure of chromosomes fixed and stained in iron-acetocarmine (see American Naturalist, 1921, pp. 573–574), where the limit of resolution in the microscope must be maintained, it has been determined that water is, on the whole, to be preferred to cedar oil as an immersion fluid for the condenser. The corrections necessary are readily made, (I) By centring a large enough meniscus lens from a photographic camera below the condenser (Hartridge); and varying the distance of the light source, and the thickness of the object slide, until the best image of a grating close to the light source is obtained. (2) By unscrewing sufficiently the top lens or lenses of the condenser. Slides can easily be selected of approximately the required thickness. The test for aplanatism is, of course, to diaphragm the source of light until its image is equal to or smaller than the field of view, and then observe the light circle at the back of the objective.
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BELLING, J. On the Daily Use of an Immersion Condenser. Nature 116, 48 (1925). https://doi.org/10.1038/116048c0
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DOI: https://doi.org/10.1038/116048c0
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