Abstract
Purified RNA polymerase initiates transcription on trp Operon DNA at or near the correct initiation site. Addition of partially purified trp repressor and tryptophan blocks this transcription.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
de Crombrugghe, B., Chen, B., Anderson, W., Nissley, P., Gottesman, M., and Pastan, I., Nature new Biol., 231, 139 (1971).
Eron, L., and Block, R., Proc. natn. Acad. Sci. U.S.A., 68, 1828 (1971).
Nissley, P., Anderson, W., Gottesman, M., Perlman, R., and Pastan, I., J. biol. Chem., 246, 4671 (1971).
Cohen, G., and Jacob, F., C.r. hebd. Séanc. Acad. Sci. Paris, 248, 3490 (1959).
Morse, D. E., and Yanofsky, C., J. molec. Biol., 44, 185 (1969).
Zubay, G., Morse, D. E., Schrenk, W. J., and Miller, J. H. M., Proc. natn. Acad. Sci. U.S.A., 69, 1100 (1972).
Squires, C. L., Rose, J. K., Yanofsky, C., Huey-Lang, Y., and Zubay, G., Nature new Biol., 245, 131 (1973).
Jackson, E. N., and Yanofsky, C., J. molec. Biol, (in the press).
Hiraga, S., J. molec. Biol., 39, 159 (1969).
Doolittle, W. F., and Yanofsky, C., J. Bact., 95, 1283 (1968).
Mosteller, R. D., and Yanofsky, C., J. Bact., 105, 268 (1971).
Yanofsky, C., Horn, V., Bonner, M., and Stasiowski, S., Genetics, 69, 409 (1971).
Rose, J. K., and Yanofsky, C., J. Bact., 108, 615 (1971).
Gillespie, D., and Spiegelman, S., J. molec. Biol., 12, 829 (1965).
Burgess, R. B., J. biol. Chem., 244, 6160 (1969).
Haseltine, W., and Müller Hill, B., in Experiments in Molecular Genetics (edit, by Miller, J. H. M.), 334 (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1972).
Hradecna, Z., and Szybalski, W., Virology, 32, 633 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ROSE, J., SQUIRES, C., YANOFSKY, C. et al. Regulation of in vitro Transcription of the Tryptophan Operon by Purified RNA Polymerase in the Presence of Partially Purified Repressor and Tryptophan. Nature New Biology 245, 133–137 (1973). https://doi.org/10.1038/newbio245133a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio245133a0
This article is cited by
-
Mutants in position 69 of the Trp repressor ofEscherichia coli K12 with altered DNA-binding specificity
Molecular and General Genetics MGG (1996)
-
The possible roles of residues 79 and 80 of the Trp repressor from Escherichia coli K-12 in trp operator recognition
Molecular and General Genetics MGG (1995)
-
Study ofl-tryptophan corepressor binding to mutatedE. coli tryptophan repressor proteins by optically detected triplet-state magnetic resonance
Journal of Fluorescence (1994)
-
A mathematical model for the regulation of tryptophan promoter
Korean Journal of Chemical Engineering (1994)
-
The structure of trp pseudorepressor at 1.65Å shows why indole propionate acts as a trp 'inducer'
Nature (1988)
