Browse Articles

  • Article |

    Physiological modelling of first-pass metabolism using data from a robotic instrument that fluidically links relevant organ chips predicts human pharmacokinetic parameters for orally administered nicotine and for intravenously injected cisplatin.

    • Anna Herland
    • , Ben M. Maoz
    • , Debarun Das
    • , Mahadevabharath R. Somayaji
    • , Rachelle Prantil-Baun
    • , Richard Novak
    • , Michael Cronce
    • , Tessa Huffstater
    • , Sauveur S. F. Jeanty
    • , Miles Ingram
    • , Angeliki Chalkiadaki
    • , David Benson Chou
    • , Susan Marquez
    • , Aaron Delahanty
    • , Sasan Jalili-Firoozinezhad
    • , Yuka Milton
    • , Alexandra Sontheimer-Phelps
    • , Ben Swenor
    • , Oren Levy
    • , Kevin K. Parker
    • , Andrzej Przekwas
    •  & Donald E. Ingber
  • Article |

    A system employing liquid-handling robotics and an integrated mobile microscope enables the automated culture, sample collection and in situ microscopy imaging of up to ten fluidically coupled organ chips within a standard tissue-culture incubator.

    • Richard Novak
    • , Miles Ingram
    • , Susan Marquez
    • , Debarun Das
    • , Aaron Delahanty
    • , Anna Herland
    • , Ben M. Maoz
    • , Sauveur S. F. Jeanty
    • , Mahadevabharath R. Somayaji
    • , Morgan Burt
    • , Elizabeth Calamari
    • , Angeliki Chalkiadaki
    • , Alexander Cho
    • , Youngjae Choe
    • , David Benson Chou
    • , Michael Cronce
    • , Stephanie Dauth
    • , Toni Divic
    • , Jose Fernandez-Alcon
    • , Thomas Ferrante
    • , John Ferrier
    • , Edward A. FitzGerald
    • , Rachel Fleming
    • , Sasan Jalili-Firoozinezhad
    • , Thomas Grevesse
    • , Josue A. Goss
    • , Tiama Hamkins-Indik
    • , Olivier Henry
    • , Chris Hinojosa
    • , Tessa Huffstater
    • , Kyung-Jin Jang
    • , Ville Kujala
    • , Lian Leng
    • , Robert Mannix
    • , Yuka Milton
    • , Janna Nawroth
    • , Bret A. Nestor
    • , Carlos F. Ng
    • , Blakely O’Connor
    • , Tae-Eun Park
    • , Henry Sanchez
    • , Josiah Sliz
    • , Alexandra Sontheimer-Phelps
    • , Ben Swenor
    • , Guy Thompson II
    • , George J. Touloumes
    • , Zachary Tranchemontagne
    • , Norman Wen
    • , Moran Yadid
    • , Anthony Bahinski
    • , Geraldine A. Hamilton
    • , Daniel Levner
    • , Oren Levy
    • , Andrzej Przekwas
    • , Rachelle Prantil-Baun
    • , Kevin K. Parker
    •  & Donald E. Ingber
  • Article |

    A vascularized human bone-marrow-on-a-chip improves the maintenance of patient-derived CD34+ cells, and recapitulates clinically relevant aspects of bone marrow injury as well as key haematopoietic defects of patients with a rare genetic disorder.

    • David B. Chou
    • , Viktoras Frismantas
    • , Yuka Milton
    • , Rhiannon David
    • , Petar Pop-Damkov
    • , Douglas Ferguson
    • , Alexander MacDonald
    • , Özge Vargel Bölükbaşı
    • , Cailin E. Joyce
    • , Liliana S. Moreira Teixeira
    • , Arianna Rech
    • , Amanda Jiang
    • , Elizabeth Calamari
    • , Sasan Jalili-Firoozinezhad
    • , Brooke A. Furlong
    • , Lucy R. O’Sullivan
    • , Carlos F. Ng
    • , Youngjae Choe
    • , Susan Marquez
    • , Kasiani C. Myers
    • , Olga K. Weinberg
    • , Robert P. Hasserjian
    • , Richard Novak
    • , Oren Levy
    • , Rachelle Prantil-Baun
    • , Carl D. Novina
    • , Akiko Shimamura
    • , Lorna Ewart
    •  & Donald E. Ingber
  • Article |

    A method that quantifies signal reductions in proton magnetic resonance spectroscopy resulting from the replacement of 1H with 2H after the administration of a deuterated substrate can be used to monitor the turnover of cellular metabolites in vivo.

    • Laurie J. Rich
    • , Puneet Bagga
    • , Neil E. Wilson
    • , Mitchell D. Schnall
    • , John A. Detre
    • , Mohammad Haris
    •  & Ravinder Reddy
  • Comment |

    As an intermediary between cells and scaffolding biomaterials, the extracellular matrix secreted by the cells offers challenges and opportunities for the design and fabrication of engineered tissues.

    • Ulrich Blache
    • , Molly M. Stevens
    •  & Eileen Gentleman
  • Editorial |

    The editing of single DNA bases in the genome is being optimized for higher editing precision and versatility.

  • News & Views |

    Negatively charged nanoparticles of about 50 nm in size permit the oral delivery of insulin and other peptide drugs by temporally enhancing the permeability of the intestinal wall.

    • Per Artursson
    •  & Patrik Lundquist
  • Article |

    Optimized adeno-associated viruses delivering split cytosine base editors and adenine base editors with trans-splicing inteins can edit brain, liver, retina, heart and skeletal-muscle tissues at therapeutically relevant efficiencies.

    • Jonathan M. Levy
    • , Wei-Hsi Yeh
    • , Nachiket Pendse
    • , Jessie R. Davis
    • , Erin Hennessey
    • , Rossano Butcher
    • , Luke W. Koblan
    • , Jason Comander
    • , Qin Liu
    •  & David R. Liu
  • News & Views |

    In an adult mouse model of tyrosinaemia, a base editor correcting an A-to-G splice-site mutation in the Fah gene restores the translation of the functional enzyme, promoting the repopulation of the liver with the corrected cells.

    • Kiran Musunuru
  • News & Views |

    An injectable biomaterial vaccine encapsulating antigens associated with acute myeloid leukaemia, dendritic-cell-targeting pro-inflammatory cytokines and an adjuvant protects mice from the disease.

    • Guojun Chen
    •  & Zhen Gu
  • News & Views |

    The therapeutic dose of small interfering RNA can be reduced by endogenously expressing and packaging the RNA into extracellular vesicles through its integration with the backbone of a highly enriched pre-microRNA.

    • Monique A. J. van Eijndhoven
    • , S. Rubina Baglio
    •  & D. Michiel Pegtel
  • News & Views |

    Large quantities of extracellular vesicles produced via cellular nanoporation, and loaded with endogenously transcribed therapeutic mRNAs and targeting peptides, boost therapeutic outcomes in vivo.

    • Antje M. Zickler
    •  & Samir EL Andaloussi
  • Article |

    A comparison of compatibilities in protospacer adjacent motifs and of on-target and off-target activities of Streptococcus pyogenes Cas9 variants at endogenous sites in human cells enables the editing of new genomic sites associated with genetic diseases.

    • Hui Kwon Kim
    • , Sungtae Lee
    • , Younggwang Kim
    • , Jinman Park
    • , Seonwoo Min
    • , Jae Woo Choi
    • , Tony P. Huang
    • , Sungroh Yoon
    • , David R. Liu
    •  & Hyongbum Henry Kim
  • News & Views |

    The overexpression of the transcription factor c-Jun improves chimeric-antigen-receptor T-cell functionality and enhances the killing of low-antigen-expressing liquid and solid cancers in mice.

    • Martin W. LaFleur
    • , Brian C. Miller
    •  & Arlene H. Sharpe
  • Article |

    Structural and functional analyses of the remodelling of intact lymphatic vasculature after the ligation of a hind-limb lymphatic vessel in sheep reveal the adaptations to the changes in fluid load that occur after lymphatic injury.

    • Tyler S. Nelson
    • , Zhanna Nepiyushchikh
    • , Joshua S. T. Hooks
    • , Mohammad S. Razavi
    • , Tristan Lewis
    • , Cristina C. Clement
    • , Merrilee Thoresen
    • , Matthew T. Cribb
    • , Mindy K. Ross
    • , Rudolph L. Gleason Jr
    • , Laura Santambrogio
    • , John F. Peroni
    •  & J. Brandon Dixon
  • News & Views |

    A deep-learning algorithm trained with retinal images and subject metadata from the UK Biobank predicts blood-haemoglobin levels.

    • Yih-Chung Tham
    • , Ching Yu Cheng
    •  & Tien Yin Wong
  • Article |

    An optical-imaging instrument that integrates a visible multispectral imaging system with the detection of near-infrared fluorescence in the first and second windows aids the fluorescence-guided surgical resection of liver tumours in patients.

    • Zhenhua Hu
    • , Cheng Fang
    • , Bo Li
    • , Zeyu Zhang
    • , Caiguang Cao
    • , Meishan Cai
    • , Song Su
    • , Xingwang Sun
    • , Xiaojing Shi
    • , Cong Li
    • , Tiejun Zhou
    • , Yuanxue Zhang
    • , Chongwei Chi
    • , Pan He
    • , Xianming Xia
    • , Yue Chen
    • , Sanjiv Sam Gambhir
    • , Zhen Cheng
    •  & Jie Tian
  • Article |

    Machine-learning algorithms trained with retinal fundus images, with subject metadata or with both data types, predict haemoglobin concentration with mean absolute errors lower than 0.75 g dl–1 and anaemia with areas under the curve in the range of 0.74–0.89.

    • Akinori Mitani
    • , Abigail Huang
    • , Subhashini Venugopalan
    • , Greg S. Corrado
    • , Lily Peng
    • , Dale R. Webster
    • , Naama Hammel
    • , Yun Liu
    •  & Avinash V. Varadarajan
  • Article |

    A cellular-nanoporation method produces large quantities of exosomes containing therapeutic mRNAs and targeting peptides that restore tumour-suppressor function in mice with orthotopically implanted phosphatase and tensin homologue (PTEN)-deficient brain gliomas.

    • Zhaogang Yang
    • , Junfeng Shi
    • , Jing Xie
    • , Yifan Wang
    • , Jingyao Sun
    • , Tongzheng Liu
    • , Yarong Zhao
    • , Xiuting Zhao
    • , Xinmei Wang
    • , Yifan Ma
    • , Veysi Malkoc
    • , Chiling Chiang
    • , Weiye Deng
    • , Yuanxin Chen
    • , Yuan Fu
    • , Kwang J. Kwak
    • , Yamin Fan
    • , Chen Kang
    • , Changcheng Yin
    • , June Rhee
    • , Paul Bertani
    • , Jose Otero
    • , Wu Lu
    • , Kyuson Yun
    • , Andrew S. Lee
    • , Wen Jiang
    • , Lesheng Teng
    • , Betty Y. S. Kim
    •  & L. James Lee
  • News & Views |

    Functional changes in leukocytes isolated from 50-μL samples of peripheral blood from sepsis patients correlate with the patients’ clinical status.

    • Daniel Irimia
  • News & Views |

    A non-invasive biomarker of the degree of collective order of monolayers of corneal endothelial cells cultured for transplantation predicts the long-term prognosis of corneal restoration in patients.

    • Jodhbir S. Mehta
    • , Viridiana Kocaba
    •  & Gary S. Peh
  • News & Views |

    The simultaneous removal of endogenous T-cell receptor α-chains and β-chains, and the orthotopic placement of an exogenous receptor in human T cells via CRISPR gene-editing, prevents the mispairing between endogenous and transgenic receptors while preserving the cells’ function.

    • Dian Kortleve
    • , Dora Hammerl
    •  & Reno Debets
  • News & Views |

    In injured intervertebral discs, disruptions in fibre organization and in cellular contractility result in a fibrotic phenotype and progressive tissue degeneration.

    • Tyler J. DiStefano
    • , Svenja Illien-Jünger
    •  & James C. Iatridis
  • Editorial |

    The physical properties of living tissues are a compelling source of biomarkers of health and disease.

  • Article |

    A near-infrared-light-sensitive photovoltaic subretinal prosthesis triggers behavioural responses in awake macaques, even two years after device implantation.

    • Paul-Henri Prévot
    • , Kevin Gehere
    • , Fabrice Arcizet
    • , Himanshu Akolkar
    • , Mina A. Khoei
    • , Kévin Blaize
    • , Omar Oubari
    • , Pierre Daye
    • , Marion Lanoë
    • , Manon Valet
    • , Sami Dalouz
    • , Paul Langlois
    • , Elric Esposito
    • , Valérie Forster
    • , Elisabeth Dubus
    • , Nicolas Wattiez
    • , Elena Brazhnikova
    • , Céline Nouvel-Jaillard
    • , Yannick LeMer
    • , Joanna Demilly
    • , Claire-Maëlle Fovet
    • , Philippe Hantraye
    • , Morgane Weissenburger
    • , Henri Lorach
    • , Elodie Bouillet
    • , Martin Deterre
    • , Ralf Hornig
    • , Guillaume Buc
    • , José-Alain Sahel
    • , Guillaume Chenegros
    • , Pierre Pouget
    • , Ryad Benosman
    •  & Serge Picaud
  • Article |

    The local delivery of a calcium channel blocker and a Rho-kinase inhibitor, determined by screening for small molecules that cause ureteral relaxation, significantly reduce ureteral contraction in pigs.

    • Christopher X. Lee
    • , Jaime H. Cheah
    • , Christian K. Soule
    • , Huiming Ding
    • , Charles A. Whittaker
    • , Kyle Karhohs
    • , Aurora A. Burds
    • , Kriti S. Subramanyam
    • , Anne E. Carpenter
    • , Brian H. Eisner
    •  & Michael J. Cima
  • Article |

    A wireless device designed to be conformally placed on the suprasternal notch can continuously provide real-time information of essential vital signs as well as talking time, swallow counts and sleep patterns.

    • KunHyuck Lee
    • , Xiaoyue Ni
    • , Jong Yoon Lee
    • , Hany Arafa
    • , David J. Pe
    • , Shuai Xu
    • , Raudel Avila
    • , Masahiro Irie
    • , Joo Hee Lee
    • , Ryder L. Easterlin
    • , Dong Hyun Kim
    • , Ha Uk Chung
    • , Omolara O. Olabisi
    • , Selam Getaneh
    • , Esther Chung
    • , Marc Hill
    • , Jeremy Bell
    • , Hokyung Jang
    • , Claire Liu
    • , Jun Bin Park
    • , Jungwoo Kim
    • , Sung Bong Kim
    • , Sunita Mehta
    • , Matt Pharr
    • , Andreas Tzavelis
    • , Jonathan T. Reeder
    • , Ivy Huang
    • , Yujun Deng
    • , Zhaoqian Xie
    • , Charles R. Davies
    • , Yonggang Huang
    •  & John A. Rogers
  • Article |

    The serial assessment of the functional parameters of leukocytes isolated via an inertial microfluidic system from 50 μl of peripheral blood from sepsis patients provides significantly more prognostic information than leukocyte counting.

    • Bakr Jundi
    • , Hyunryul Ryu
    • , Do-Hyun Lee
    • , Raja-Elie E. Abdulnour
    • , Braden D. Engstrom
    • , Melody G. Duvall
    • , Angelica Higuera
    • , Mayra Pinilla-Vera
    • , Maura E. Benson
    • , Jaemyon Lee
    • , Nandini Krishnamoorthy
    • , Rebecca M. Baron
    • , Jongyoon Han
    • , Joel Voldman
    •  & Bruce D. Levy
  • News & Views |

    A data-clustering method that incorporates prior knowledge of biological context reveals prognostic signatures of proteomic expression in patients with acute myeloid leukaemia.

    • Matthew A. Clarke
    •  & Jasmin Fisher
  • News & Views |

    Personalized computational modelling of optimal ablation targets on heart tissue guides the treatment of patients with persistent atrial fibrillation.

    • Hui-Nam Pak
    •  & Byounghyun Lim
  • Perspective |

    This Perspective summarizes the most recent developments in the bioprinting of cartilage, bone and skin for clinical applications, and discusses translational challenges and opportunities in the fabrication of complex 3D-printed tissues and organs.

    • Sean V. Murphy
    • , Paolo De Coppi
    •  & Anthony Atala
  • Author Correction |

    • Mohammed F. Shaheen
    • , Dong Jin Joo
    • , Jeffrey J. Ross
    • , Brett D. Anderson
    • , Harvey S. Chen
    • , Robert C. Huebert
    • , Yi Li
    • , Bruce Amiot
    • , Anne Young
    • , Viviana Zlochiver
    • , Erek Nelson
    • , Taofic Mounajjed
    • , Allan B. Dietz
    • , Gregory Michalak
    • , Benjamin G. Steiner
    • , Dominique Seetapun Davidow
    • , Christopher R. Paradise
    • , Andre J. van Wijnen
    • , Vijay H. Shah
    • , Mengfei Liu
    •  & Scott L. Nyberg
  • Article |

    Attaching a syndecan-binding domain to vascular endothelial and platelet-derived growth factor variants enhances their binding to syndecans and triggers tonic signalling for enhanced bone regeneration and wound repair in mice.

    • Mayumi Mochizuki
    • , Esra Güç
    • , Anthony J. Park
    • , Ziad Julier
    • , Priscilla S. Briquez
    • , Gisela A. Kuhn
    • , Ralph Müller
    • , Melody A. Swartz
    • , Jeffrey A. Hubbell
    •  & Mikaël M. Martino
  • Article |

    A computational approach for the design of self-assembling antibody–drug conjugates via affinity ligands for specific conserved amino acid residues in antibodies yields homogenous and stable antibody–drug conjugates that form in less than 8 min.

    • Nimish Gupta
    • , Aasif Ansari
    • , Gaurao V. Dhoke
    • , Maheshwerreddy Chilamari
    • , Jwala Sivaccumar
    • , Smita Kumari
    • , Snigdha Chatterjee
    • , Ravinder Goyal
    • , Pradip Kumar Dutta
    • , Mallik Samarla
    • , Madhumita Mukherjee
    • , Arindam Sarkar
    • , Swadhin K. Mandal
    • , Vishal Rai
    • , Goutam Biswas
    • , Aniruddha Sengupta
    • , Sudip Roy
    • , Monideepa Roy
    •  & Shiladitya Sengupta
  • News & Views |

    Computationally designed antibody–drug conjugates easily self-assemble via affinity ligands that non-covalently link a therapeutic drug to conserved sites on native IgG antibodies.

    • Christine S. Nervig
    •  & Shawn C. Owen
  • Article |

    Functionalized multi-walled carbon nanotubes twisted into helical fibre bundles that mimic the hierarchical structure of muscle can be used for the long-term monitoring of multiple disease biomarkers in vivo.

    • Liyuan Wang
    • , Songlin Xie
    • , Zhiyuan Wang
    • , Fei Liu
    • , Yifan Yang
    • , Chengqiang Tang
    • , Xiaoying Wu
    • , Peng Liu
    • , Yongjing Li
    • , Hexige Saiyin
    • , Shuang Zheng
    • , Xuemei Sun
    • , Fan Xu
    • , Hongbo Yu
    •  & Huisheng Peng
  • News & Views |

    Applying motion correction to standard foetal magnetic resonance imaging via image-registration techniques improves the diagnostic evaluation of cardiovascular structures in foetuses.

    • Ali Gholipour
    •  & Simon K. Warfield
  • Article |

    The analysis of behavioural patterns from standardized video recordings of infants with varying degrees of visual impairment enables, via deep learning, classification of the infants by visual-impairment severity and by ophthalmological condition.

    • Erping Long
    • , Zhenzhen Liu
    • , Yifan Xiang
    • , Andi Xu
    • , Jialing Huang
    • , Xiucheng Huang
    • , Xiaoyan Li
    • , Zhuoling Lin
    • , Jing Li
    • , Jingjing Chen
    • , Yan Zhang
    • , Yi Zhu
    • , Chuan Chen
    • , Ziheng Zhou
    • , Xiaowei Ding
    • , Xiaohang Wu
    • , Wangting Li
    • , Hui Chen
    • , Ruiyang Li
    • , Yahan Yang
    • , Weishi Zheng
    • , Weirong Chen
    • , Haotian Lin
    •  & Yizhi Liu
  • Article |

    Downregulation of the transcription factor KLF4 in keratinocyte precursors improves the proliferation and maintenance of immature keratinocytes in vitro and the generation of epidermis in vivo.

    • Nicolas O. Fortunel
    • , Loubna Chadli
    • , Julien Coutier
    • , Gilles Lemaître
    • , Frédéric Auvré
    • , Sophie Domingues
    • , Emmanuelle Bouissou-Cadio
    • , Pierre Vaigot
    • , Sophie Cavallero
    • , Jean-François Deleuze
    • , Paul-Henri Roméo
    •  & Michèle T. Martin
  • Editorial |

    The discoveries of this year’s Nobel laureates in Physiology and Medicine have improved diagnostic and therapeutic strategies relying on oxygen sensing.

  • News & Views |

    A deep-learning model for cancer detection trained on a large number of scanned pathology slides and associated diagnosis labels enables model development without the need for pixel-level annotations.

    • Jeroen van der Laak
    • , Francesco Ciompi
    •  & Geert Litjens
  • News & Views |

    Haematopoietic stem and progenitor cells, edited via CRISPR to mimic mutations ameliorating sickle-cell disease, engraft when transplanted in macaques and maintain foetal haemoglobin in circulation.

    • Daniel E. Bauer
  • Article |

    Decellularized whole porcine livers revascularized with human endothelial cells and implanted heterotopically into immunosuppressed pigs whose spleens had been removed can sustain perfusion for up to 15 days.

    • Mohammed F. Shaheen
    • , Dong Jin Joo
    • , Jeffrey J. Ross
    • , Brett D. Anderson
    • , Harvey S. Chen
    • , Robert C. Huebert
    • , Yi Li
    • , Bruce Amiot
    • , Anne Young
    • , Viviana Zlochiver
    • , Erek Nelson
    • , Taofic Mounajjed
    • , Allan B. Dietz
    • , Gregory Michalak
    • , Benjamin G. Steiner
    • , Dominique Seetapun Davidow
    • , Christopher R. Paradise
    • , Andre J. van Wijnen
    • , Vijay H. Shah
    • , Mengfei Liu
    •  & Scott L. Nyberg