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Enhanced Antitumor Efficacy of Vasculostatin (Vstat120) Expressing Oncolytic HSV-1
Jayson Hardcastle, Kazuhiko Kurozumi, Nina Dmitrieva, Martin P Sayers, Sarwat Ahmad, Peter Waterman, Ralph Weissleder, E Antonio Chiocca and Balveen Kaur
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Schematic diagram of the genetic structure of wild type HSV-1, HSVQ and RAMBO. Schematic diagram of the genetic structure of wild type HSV-1, HSVQ and RAMBO. Restriction digests of RAMBO by Xho I results in the 2.55 kb and 0.3 kb uniquely cleaved fragments indicated in Figure 2 a-c.
Figure S2. (pdf 19K)
Western-blot analysis of Vstat120 expression in intracranial glioma (U87
EGFR) xenografts. Western-blot analysis of Vstat120 expression in intracranial glioma (U87?EGFR) xenografts. Tumors were treated seven days post implantation intratumorally with a single injection (1 x105 pfu) of the indicated OVs. The mice were sacrificed 24 hrs later and the lysed brains probed for Vstat120 expression. GAPDH levels were probed as a loading control.
Figure S3. (pdf 50K)
Western-blot analysis of Vstat120 expression in cell lysate, extracellular matrix (ECM) and conditioned medium (CM) of U251 glioma cells treated with PBS, HSVQ or RAMBO (MOI=2). Western-blot analysis of Vstat120 expression in cell lysate, extracellular matrix (ECM) and conditioned medium (CM) of U251 glioma cells treated with PBS, HSVQ or RAMBO (MOI=2). GAPDH levels were probed as a loading control.
Table S1. (doc 31K)
Effect of Vasculostatin production on viral replication.
