FIGURE 2

Quantification of endometriotic-like lesion size in ovariectomized mice supplemented or not with E2. Ovariectomized nude mice having received or not an E2 pellet (N = 8 for each group) were periodically imaged in triplicate, and endometriotic-like lesion size (number of pixels) was quantified by image analysis. Each symbol represents the mean of three measures for each animal at each time point. Bars indicate the mean surface of endometriotic implants for each group. Comparison of lesion size in estradiol-supplemented versus nonsupplemented animals was performed by a nonparametric Mann–Whitney test. A significant difference in size of lesions is observed between the two groups starting from day 12 (P 0.05 at days 12, 27, and 29; P 0.005 at days 13, 14, 16, 19, 21, and 23). These results are representative of six independent experiments.