Molecular Therapy

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Quantitative Assessment of Human Endometriotic Tissue Maintenance and Regression in a Noninvasive Mouse Model of Endometriosis

Marylène Fortin, Manon Lépine, Yannick Merlen, Isabelle Thibeault, Claudine Rancourt, Diane Gosselin, Patrice Hugo and Ann-Muriel Steff

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Figure 1.

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Noninvasive in vivo imaging of fluorescent endometrial implants allows dynamic monitoring of lesion development. Endometrial fragments were incubated with AdGFP viral particles for 20 h. After washings, five fragments were injected subcutaneously into ovariectomized nude mice supplemented (N = 8) or not (N = 8) with E2-releasing pellets. Each mouse was repeatedly imaged in triplicate, and images acquired at days 4, 6, 8, 12, 14, 16, 19, 23, 27, and 29 posttransplantation are shown for one mouse in each group.

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