FIGURE 5

Effects of the orientation of expression cassettes on SB HITT-vector function. HITT vectors carrying the transposase expression cassette in orientation opposite to that of the transposon-embedded hAAT cDNA (constructs labeled with bid for "bidirectional") were compared to constructs with both expression cassettes oriented in the same direction. Plasmids (30 g) were injected into the tail vein of mice and the serum hAAT concentration was monitored over time. (A) Comparison of HITT vectors with CMV-, Alb-, and PIL-directed transposase expression (n = 5). The expression profiles of the "unidirectional" vectors (indicated with large symbols), presented previously in Fig. 2B, are included for direct comparison. (B) Comparison of HITT-vector-based PGK- and MTH-directed transposase production (n = 4–5). Profiles of pT/hAAT.PGK-SB and pT/hAAT.MTH-SB, shown earlier in Fig. 4A, are included for easy comparison. Mean values standard deviation are shown.