Molecular Therapy

FIGURE 2

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Helper-Independent and AAV-ITR-Independent Chromosomal Integration of Double-Stranded Linear DNA Vectors in Mice

Hiroyuki Nakai, Eugenio Montini, Sally Fuess, Theresa A. Storm, Leonard Meuse, Milton Finegold, Markus Grompe and Mark A. Kay

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Figure 2.

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Histological analysis of hFAH-expressing vector-injected HTI mouse liver. Representative results of the FAH staining of liver sections before (A, C, E, G, and I) and after (B, D, F, H, and J) in vivo selection. (A, B) hFAH-C+; (C, D) hFAH-C-; (E, F) hFAH-L+; (G, H) hFAH-L-; (I, J) AAV-hFAH. hFAH-expressing hepatocytes were stained brown. Black arrows indicate FAH-positive hepatocyte clusters. Original magnifications, times10 and times2.5 for sections before and after selection, respectively. (K) Percentage of FAH-positive hepatocytes in the livers after in vivo selection of 18 weeks, determined by histological analysis. Vertical bars indicate standard deviations.

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