Method
Molecular Therapy (2001) 4, 372–374; doi: 10.1006/mthe.2001.0462
A Single-Step Affinity Column for Purification of Serotype-5 Based Adeno-associated Viral Vectors
Alberto Auricchio1, Erin O'Connor1, Markus Hildinger1 and James M. Wilson1
1Institute for Human Gene Therapy, Department of Molecular and Cellular Engineering, University of Pennsylvania and The Wistar Institute, Philadelphia, Pennsylvania, 19104, USA
Correspondence: James M. Wilson, Fax: (215) 898-6588. E-mail: wilsonjm@mail.med.upenn.edu.
Received 21 June 2001; Accepted 2 August 2001.
Abstract
Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl2 sedimentation.
Keywords:
AAV5 purification, sialic acid, mucin, affinity column
