FIGURE 6
FROM:
Injection of Recombinant Human Type VII Collagen Corrects the Disease Phenotype in a Murine Model of Dystrophic Epidermolysis Bullosa
Jennifer Remington, Xinyi Wang, Yingpin Hou, Hui Zhou, Julie Burnett, Trevor Muirhead, Jouni Uitto, Douglas R Keene, David T Woodley and Mei Chen
BACK TO ARTICLEFigure 6.
Anti-type VII collagen (anti-C7) IgG production in dystrophic epidermolysis bullosa mice after protein therapy. (a) Anti-C7 IgG production was measured by NC1-based enzyme-linked immunosorbent assay over time after intradermal injetions with recombinant human C7. Anti-C7 IgG was detected at 30 days and increased steadily thereafter in C7-treated mice (mean 
SD, n = 6). (b) Immunolabeling of mouse and human skin with serum from mice injected with C7. Sections of normal mouse skin (A) and salt-split human skin (B) were stained with mouse serum obtained from mice injected with C7 at a dilution of 1:20. Note that circulating antibodies labeling the basement membrane zone of mouse skin and the dermal floor of salt-split human skin were found in the serum samples from C7-injected mice. OD, optical density.
