Molecular Therapy

FIGURE 4

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Artificial MicroRNAs as siRNA Shuttles: Improved Safety as Compared to shRNAs In vitro and In vivo

Ryan L Boudreau, Inês Martins and Beverly L Davidson

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Figure 4.

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Effects of shSCA1 and miSCA1 on cell viability in vitro. C2C12 cells were transfected with RNAi plasmids co-expressing hrGFP, and differentiated. Photomicrographs depicting hrGFP expression at 24 and 72 h after treatment are shown. Cell viability was measured by CellTiter-96 AQueous MTS assay (Promega, n = 3, mean values plusminus SEM).

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