FIGURE 2

Podosome recovery and interference reflection microscopy (IRM) on bone marrow dendritic cells (DCs). (a) Bone marrow–derived dendritic cells (BMDCs) containing podosomes had the number of podosomes per cell counted. Each point represents an individual cell. Fifty to two hundred cells were counted. (b) BMDCs were plated onto glass coverslips and confocal microscopy images captured to reveal the actin cytoskeleton. Arrows point to podosomes. (c) SEWW, (d) SEW, and (e) SEW WT BMDCs were imaged by time-lapse microscopy capturing both the enhanced green fluorescent protein (eGFP) fluorescence and IRM images. Panels show the movement over a time frame of 6.5 minutes, with the final cell position outlined in red over the starting image. (f) Turnover index of adhesion contacts in transduced and control DCs. ^* P < 0.05 (Students t-test) SEWW 1°SEWW 2° and SEW WT compared to WAS KO and SEW DCs and error shown is SEM. n = 5–18. KO, knockout; WAS, Wiskott–Aldrich syndrome; WT, wild-type.