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Gene Transfer to Ocular Stem Cells by Early Gestational Intraamniotic Injection of Lentiviral Vector
Masayuki Endo, Philip W Zoltick, Daniel C Chung, Jean Bennett, Antoneta Radu, Nidal Muvarak and Alan W Flake
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Schematic view of a developing murine eye and the exposure of ectodermal tissues to amniotic fluid at different gestational stages. The figure illustrates the progressive steps in eye development and the relation between the timing of injection and the distribution of exogenous gene injected into the amniotic fluid. The three important events related to the eye are (1) Neural tube closure between E8 to E9, (2) lens pit closure between E10 and E11, and (3) eyelid fusion between E16 and E17. Before neural tube closure, at E8, both the neural ectoderm-derived tissues (retina, iris, and optic nerve) and the surface ectoderm-derived tissues (lens and cornea) are accessible for IAGT as documented by our observed distribution of transduction. After the neural tube closure and before the lens pit closure, the cornea and the lens are transduced by IAGT. After the lens pit closure and before the eyelids fuse, only the cornea is transduced by IAGT. After eyelid fusion occurs, the eye is no longer accessible to IAGT.