TABLE 1
FROM:
Complete Correction of Enzymatic Deficiency and Neurochemistry in the GM1-gangliosidosis Mouse Brain by Neonatal Adeno-associated Virus–mediated Gene Delivery
M L D Broekman, R C Baek, L A Comer, J L Fernandez, T N Seyfried and M Sena-Esteves
BACK TO ARTICLETable 1. Effect of AAV vector on the concentration of lipids in the brain of adult 
gal mice
| Strain | Treatment | N a | Total gangliosides | GM1 b | GD1a b | GT1a b | GD1b b | GT1b b | GQ1b b | GA1 | Cholesterol c | Cerebrosides c | Sulfatides c |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C57BL/6 | — | 3 | 412 17 | 531 | 1644 | 141 | 291 | 1188 | 344 | Not detectable | 6.90.3 | 2.70.1 | 0.810.14 |
 gal-/-
| — | 3 | 82465d
| 47642d
| 15312 | 161 | 401d
| 1107 | 304 | 1.640.05 | 6.60.3 | 1.60.2e
| 0.310.04e
|
|
gal-/-
| AAV | 7 | 44421 | 472 | 1699 | 171 | 321 | 1366 | 433 | Not detectable | 7.40.4 | 3.00.3 | 0.820.09 |
AAV, adeno-associated virus.
a N, the number of independent mice analyzed.
b Ganglioside concentrations were expressed as microgram sialic acid/100 mg dry weight. Concentration of GA1 was expressed as mg/100 mg dry weight. Values represent the mean1SEM. Gangliosides were identified according to the nomenclature system of Svennerholm.43 Gangliosides that comprised less than 1% of the total ganglioside content were not included (GM2, GM3, and GM4). Determined from densitometric scanning of HPTLC plates as shown in Figure 3a and b.
c Lipid concentrations expressed as mg/100 mg dry weight. Values represent the mean1SEM. Determined from densitometric scanning of HPTLC plates as shown in Figure 6.
d Indicates that the concentration is significantly different from that in wild-type C57BL/6 and AAV-treated gal-/- mice at P<0.01 by one-way ANOVA.
e Indicates that the concentration is significantly different from that in wild-type C57BL/6 controls and AAV-treated gal-/- mice at P<0.05 by one-way ANOVA.
