FIGURE 3
FROM:
Transcriptional Activation of Gene Expression by Pluronic Block Copolymers in Stably and Transiently Transfected Cells
Srikanth Sriadibhatla, Zhihui Yang, Catherine Gebhart, Valery Yu Alakhov and Alexander Kabanov
BACK TO ARTICLEFigure 3.
Effects of Pluronics on mRNA levels of luciferase (Luc) and hsp68 genes in LucCMV-NIH3T3 cells. Cells were treated with 0.1% L64 or 1% P85 for 3 h, washed, and incubated in complete medium for an additional 24 h. (A) The RT-PCR products of Luc (230 bp) and hsp68 (664 bp) were run by electrophoresis on a 2% agarose gel containing ethidium bromide. The image was acquired using an Alpha Imager and analyzed using ImageQuant. (B) mRNA levels for hsp68 and luciferase normalized with respect to GAPDH and expressed as arbitrary units. (C) mRNA levels for luciferase relative to GAPDH quantitated by RT2-PCR. Data are reported as means 
SD (n = 3). Differences in treated versus control groups were considered significant at *
P 
0.05, **
P 0.005.
