FIGURE 2

Effects of Pluronics on GFP expression. (A) Mean fluorescence values for GFP^CMV-Cl66 cells treated with P85 for 3, 6, and 9 h. (B) Mean fluorescence values for GFP^CMV-NIH3T3 cells treated with 1% P85 or 0.1% L64 for 3 h. (A, B) Data are reported as means SD (n = 3). The statistical comparisons were made for treated versus untreated cells: ^* P < 0.05; ^** P < 0.005. (C, D) Confocal fluorescence micrographs of (C) untreated GFP^CMV-C2C12 cells and (D) GFP^CMV-C2C12 cells treated with 0.1% L64 for 3 h. The micrographs superimpose nonconfocal differential interference contrast and confocal images collected simultaneously. A similar increase in gene expression by 0.1% L64 with no changes in cell morphology was observed in GFP^CMV-NIH3T3 cells (not shown).