FIGURE 3
FROM:
Prolonged transgene expression in murine salivary glands following non-primate lentiviral vector transduction
Ela Shai, Aaron Palmon, Amos Panet, Yitzhak Marmary, Yoav Sherman, Michael A. Curran, Eithan Galun and Reba Condiotti
BACK TO ARTICLEFigure 3.
Histochemistry of salivary glands for the assessment of cell types transduced by FIV-based vectors. Mice were administered FIV-
-gal, and at days 3 (A), 15 (B), and 29 (C) posttransduction the salivary glands were removed and a whole-mount staining was performed using X-gal. The paraffin-embedded sections were stained with H&E and counterstained with nuclear fast red. From day-29 samples, serial sections were made, and one was stained as above (D), whereas the sequential section was stained with Alcian blue (E). Magnification, 
400. (F) Immunofluorescence labeling was performed with anti-GFP and FITC-conjugated secondary antibodies on cryostat sections of murine salivary glands removed 50 days following transduction with FIV-YFP. The cells were counterstained with DAPI, which stains cell nuclei a fluorescent blue.
