FIGURE 3
FROM:
A Novel Bovine Virus Efficiently Transduces Inner Ear Neuroepithelial Cells
Giovanni Di Pasquale, Agnieszka Rzadzinska, Mark E. Schneider, Ioannis Bossis, John A. Chiorini and Bechara Kachar
BACK TO ARTICLEFigure 3.
Transduction is continuous and time-dependent. (A) Confocal image of vestibular epithelia after 5 days of BAAV infection. The positively transduced hair cells are easily scored even though the yield is sparse. (B) Confocal image of vestibular epithelia after 8 days of BAAV transduction demonstrating that almost half of the hair cells are transduced. There is a statistically significant increase (n = 7 and 10 frames, respectively, from at least three explants, P > 0.01) in the transduced cells after an additional 3 days of incubation. (C) Gene gun-mediated 
-actin–GFP incorporation in stereocilia after 8 h. The yield of transfected hair cells is one or two per vestibular epithelia explant and hair bundles are frequently damaged. (D) The initiation of -actin–GFP incorporation is asynchronous in BAAV-transduced epithelia. The extent of actin incorporation into stereocilia varies from just the tips of a hair bundle labeled (arrows) to the entire bundle (arrowheads). (Bar, 10 
m.)
