Original Article
Molecular Therapy (2006) 11, 142–148; doi: 10.1016/j.ymthe.2004.08.021
Development of Ecdysone-Regulated Lentiviral Vectors
Francesco Galimi1, Enrique Saez2, Jason Gall1, Nien Hoong1, Gerald Cho2, Ronald M. Evans2 and Inder M. Verma1
- 1Laboratory of Genetics, 10110 North Torrey Pines Road, La Jolla, CA 92037, USA
- 2Gene Expression Laboratory, H.H.M.I., The Salk Institute for Biological Studies, 10110 North Torrey Pines Road, La Jolla, CA 92037, USA
Correspondence: Inder M. Verma, E-mail: verma@salk.edu
Received 9 March 2004; Accepted 9 August 2004.
Abstract
We have engineered a lentivirus-based gene transfer system to achieve ecdysone-regulated transgene expression. The method combines the wide tropism of lentiviral vectors and the possibility of gene regulation by a small molecule with an excellent pharmacological profile. Using the hematopoietic tissue as a model, we transduced mouse progenitors with an ecdysone-regulated GFP expression cassette. The ecdysone gene switch efficiently turned GFP on and off in transplanted animals, showing low basal activity. This system allows the delivery of inducible transcriptional units in vitro and ex vivo and may be a useful tool for gene transfer purposes. Moreover, our work provides hints on the design of lentiviral vectors containing multiple expression cassettes with multiple promoters.
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