Supplementary information
FROM:
Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis
Maria Piques, Waltraud X Schulze, Melanie Höhne, Björn Usadel, Yves Gibon, Johann Rohwer & Mark Stitt
doi:10.1038/msb.2009.68
BACK TO ARTICLEGeneral supplementary information
Supplemental Figures 1-3
Supplementary table 2
Transcript levels in unfractionated extracts and polysome gradient fractions, and estimation of rates of translation
Supplementary table 4
Literature information about enzymes
Supplementary table 5
Protein abundance and Tp estimated from enzyme activities
Supplementary table 6
Protein abundance estimated from quantitative proteomics
Supplementary table 7
Protein abundance and Tp for enzymes of primary metabolism estimated from quantitative proteomics
Supplementary table 8
Empai values for all proteins identified in the different ribosomal fractions (NPS, SPS, LPS) in dark and 2h light for three biological replica sets
Supplementary table 9
List of all unique peptides with score above the 95% significance cutoff (score >32) identified in the different ribosomal fractions (NPS; SPS; LPS) from three biological replicates and the two time points (dark=0 and 2h light=2)
Supplementary table 10
This table contains for each gene the estimated CAI and Nc,the length of the open reading frame, the GC content of the entire open reading frame and of the codon positon 3, the mean copy number of the transcript in the dark and in the light, and the ribosomal occupancy [(SPS+LPS)/(NPS+SPS+LPS)
Supplementary table 11
Spearmens regression coefficient for correlations between CAI, Nc, the average transcript abundance in the dark and in the light, polysome loading in the dark and the light, the open reading frame length, and GC content of the entire open reading frame, and at positons 1, 2 and 3 of codons
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