FIGURE 7 

Blocking of protein synthesis in early and late phases. Simulations are implemented with and without a (Ca²⁺)₄–CaM pulse. The solid line indicates the CaMKII concentration without any pulse stimulus, whereas the dotted line indicates the CaMKII concentration with pulse stimulus (pulse stimulus is used to mimic the effect of HFS). (A) Protein synthesis blocking during induction for 33 min (solid thick black line shows blocking time). For different levels of blocking, L-LTP has a different outcome during the maintenance phase. (B) Protein synthesis blocking during maintenance for 1300 min (the solid thick black line shows blocking time). Even for high-percentage blocking, the upregulated state can still be maintained, suggesting that if CaMKII is a trace for L-LTP, its expression can still be observed, even if synthesis of new proteins is blocked.