FIGURE 2 

Analysis of TAP-tagged PSD-95 localization and synaptic plasticity in PSD-95^TAP/TAP mice. (A) Immunohistochemical staining of PSD-95 in sagittal brain sections from PSD-95^TAP/TAP and wt mice. B, brainstem; C, cortex; CB, cerebellum; H, hippocampus; S, striatum. Scale bar=1 mm. (B) Immunohistochemical staining of PSD-95 in sagittal hippocampus sections from PSD-95^TAP/TAP and wt mice showing CA1, CA3 and dentate gyrus (DG). Scale bar=1 mm. (C) Synaptic localization of TAP-tagged PSD-95 in primary hippocampus neurons. DIV14 neurons from wt and PSD-95^TAP/TAP mice were stained with PSD-95 and MAP2B antibodies (top panels). Three lower panel show PSD-95 and FLAG antibody staining in a culture from PSD-95^TAP/TAP mice (bottom panels). Inset panels show higher magnification of synaptic puncta labeling with each antibody and merged image. Scale bar=10 m. (D) Long-term potentiation of fEPSPs induced by theta-burst stimulation in CA1 area of hippocampal slices is similar in PSD-95^TAP/TAP (13 slices from 4 animals) and wild-type mice (15 slices from 4 animals).