"Flow cytometry data for different base strains (NBY157, NBY195-199) transformed with different promoters (CYC1,ADH1,TEF1,TDH3,ADE17,MET3,MET17,LYS9) driving CEBPa-RFP. Those transformants which (i) exhibited bimodal CEBPa-RFP distribution (i.e. colony is mixture of two transformants), (ii) aberrant FSC/SSC, (iii) no measureable CEBPa-RFP were discarded and are not contained in this data set. All good transformants were grown in SCD medium except for those containing amino-acid repressed promoters, which were grown in SCD-AA (amino-acid depeleted) or SCD+10xAA (saturating amounts of amino-acid). " Data Name FSC-H(Mean) SSC-H(Mean) 561-H(Mean) 488-H(Mean) 561-H(CV) 488-H(CV) RedConc(Mean) YelConc(Mean) RedConc(CV) YelConc(CV) BASE STRAIN - PROMOTER - TRANSFORMANT - GROWTH CONDITION Forward Scatter Side scatter "Total, red fluoresence" "Total, yellow fluorescence" Coefficient variation of total red fluorescence (%) Coefficient of variation of total yellow fluorescence (%) Red fluorescent pseudoconcentration (10000*561-H/FSC-H) Yellow fluorescent pseudoconcentration (10000*488-H/FSC-H) Coefficient of variation of RedConc Coefficient of variation of YelConc NBY157-ADE17-10+10xADE 65991 49485 370 352 127 55.5 56.9 52.8 134 48.7 NBY157-ADE17-10-ADE 63481 49775 3176 784 36.8 43.6 508 123 34.9 35.5 NBY157-ADE17-11+10xADE 65297 48644 380 359 71.1 53 58.8 54.3 65 44 NBY157-ADE17-11-ADE 63220 50492 3350 766 32.3 41.9 539 120 30.8 32.8 NBY157-ADE17-2+10xADE 67162 49386 244 237 92.2 55.7 37.3 35 94.7 48.1 NBY157-ADE17-2-ADE 64862 50387 1624 716 39.8 42.5 254 110 38.5 34.5 NBY157-ADE17-3+10xADE 66022 48819 510 431 117 53.7 78.1 64.4 120 46.1 NBY157-ADE17-3-ADE 62897 49056 4789 777 33 41.7 774 123 31.7 34.4 NBY157-ADE17-9+10xADE 65297 48956 366 337 59.5 53.2 56.8 51 57.9 44.7 NBY157-ADE17-9-ADE 62416 48814 3098 713 34.1 42.4 504 114 32 34.2 NBY157-ADH1-11 64287 47935 4140 722 41.2 52 648 111 37.5 43.4 NBY157-ADH1-12 64354 49389 3839 752 40.8 49.2 598 115 37.1 40.2 NBY157-ADH1-2 65330 49214 3746 756 38.8 48.6 576 114 35 38.9 NBY157-ADH1-3 66024 48538 3985 735 42.1 49.9 610 110 38.9 41 NBY157-ADH1-4 65147 50215 4446 766 39.4 48.6 688 116 36.1 39.8 NBY157-ADH1-6 64203 47393 4001 733 37.8 48.1 629 113 34.5 38.5 NBY157-ADH1-7 64572 48563 4055 741 36.3 46.4 633 113 32.7 36.9 NBY157-ADH1-8 64625 49051 3985 735 39.5 49.6 625 113 36.5 40.2 NBY157-CYC1-12A 68462 48078 598 407 74.3 49.6 88.4 59 70.2 41.9 NBY157-CYC1-15 70223 50117 270 228 93.5 51.3 39.4 32.2 118 46 NBY157-CYC1-16 68705 50554 1784 566 44.7 42.6 263 81.8 43.8 35.2 NBY157-CYC1-19 71509 53328 1004 526 70.9 45.1 142 73.1 79.5 40.2 NBY157-CYC1-20 69638 50082 275 240 71.5 48.9 40.4 34.3 77 41.9 NBY157-CYC1-21 69513 49450 556 422 69.2 46.1 81.4 60.2 79.5 38.8 NBY157-CYC1-22 69680 50720 272 241 57 48.8 40.1 34.6 60.2 42.7 NBY157-LEU1-10+3xLEU 67733 48523 247 297 58.7 52.9 37.4 43.3 55.5 42.4 NBY157-LEU1-10-LEU 70984 50081 4790 711 35.8 43.3 672 98.5 29.7 33.6 NBY157-LEU1-11+3xLEU 67942 48358 250 319 55.7 52.8 37.8 46.2 62.6 41.8 NBY157-LEU1-11-LEU 71079 50284 4855 750 38.5 44.3 679 104 32.4 34.9 NBY157-LEU1-12+3xLEU 67453 49088 267 332 124 54.8 40.7 48.4 126 44.6 NBY157-LEU1-12-LEU 69347 49614 4409 741 44 49.7 626 104 37.3 38.4 NBY157-LEU1-3+3xLEU 66547 48414 433 438 159 52.5 65.7 64.8 149 41.3 NBY157-LEU1-3-LEU 68939 52235 8067 632 42.4 54.3 1161 90.2 36.9 46 NBY157-LEU1-6+3xLEU 66764 47500 243 289 62.8 54.3 37.5 42.5 61.6 43 NBY157-LEU1-6-LEU 69646 49431 4655 704 37.2 45.3 666 99.4 30.9 35.2 NBY157-LEU1-8+3xLEU 67490 48650 254 284 136 59 38.4 41.4 112 47.7 NBY157-LEU1-8-LEU 70476 50224 4546 704 40.7 47.6 643 98.4 34.9 38.2 NBY157-LEU1-9+3xLEU 67169 48721 404 431 84.8 50.6 61 63 87.7 39.1 NBY157-LEU1-9-LEU 67173 50649 7782 600 48.1 56.2 1128 86.2 41.6 44.6 NBY157-LYS9-10+10xLYS 67205 48397 809 631 46.6 49.8 122 92.6 43.8 40.8 NBY157-LYS9-10-LYS 73773 55041 5794 688 39.2 46.5 782 92.1 34.1 39.1 NBY157-LYS9-11+10xLYS 68257 49235 448 504 55.4 55.7 66.5 72.7 52.2 47.1 NBY157-LYS9-11-LYS 71516 52823 2730 659 37.6 52.8 382 91.2 32.8 43.1 NBY157-LYS9-3+10xLYS 67048 48703 3793 673 54.6 51.1 567 98.8 50.7 40.5 NBY157-LYS9-3-LYS 77376 57876 20837 543 38.9 48.2 2689 69.7 35.5 42.6 NBY157-LYS9-4+10xLYS 66394 47391 1181 689 47.5 50.3 180 102 42.1 39.3 NBY157-LYS9-4-LYS 71990 53771 8092 671 37.1 47.7 1120 92 30.5 38.9 NBY157-LYS9-6+10xLYS 68044 49690 447 500 64.9 48 66.3 72.6 60.7 38.4 NBY157-LYS9-6-LYS 71853 53022 2740 682 37.4 45.2 382 93.8 32.9 37.4 NBY157-LYS9-7+10xLYS 66655 48112 829 614 47.1 46.9 126 90.9 44 36.4 NBY157-LYS9-7-LYS 71839 53688 5357 693 36.1 45.2 745 95.5 31.4 38 NBY157-LYS9-9+10xLYS 68290 49026 464 519 54.5 46.9 68.7 74.9 52.2 36.9 NBY157-LYS9-9-LYS 73045 54194 2785 712 37.3 52.8 381 96.3 31.8 47.1 NBY157-MET17-3+10xMET 67052 47573 234 255 53.3 52.6 35.7 37.5 53.7 44.4 NBY157-MET17-3-MET 56904 41903 3869 537 34.1 43.2 691 93.6 31.8 33.7 NBY157-MET17-5+10xMET 66461 46860 237 257 78 54.8 36.6 38.2 80.3 46.6 NBY157-MET17-5-MET 55952 41062 3816 509 34.3 43.5 693 90.4 31.5 34 NBY157-MET17-7+10xMET 67160 47993 244 262 54.3 51.7 37.2 38.6 54.5 43.4 NBY157-MET17-7-MET 57042 42166 4124 544 34.1 43.2 734 94.9 31.3 33.8 NBY157-MET17-8+10xMET 67006 47626 235 263 52.6 53.1 35.9 38.7 52.9 44.7 NBY157-MET17-8-MET 57199 42099 3711 555 34 43.6 660 96.3 32.1 33.9 NBY157-MET17-9+10xMET 65849 47399 411 377 74.1 48.9 63.4 56.7 64.7 39.8 NBY157-MET17-9-MET 59882 45117 9924 485 33.9 43.7 1673 80.5 29.4 34.9 NBY157-MET3-10+10xMET 64153 46054 242 196 68.4 51.8 38.7 30.2 73.3 43 NBY157-MET3-10-MET 60531 45228 10431 465 37.7 45.2 1732 76.3 32.7 36.8 NBY157-MET3-2+10xMET 68920 48763 181 147 71.1 53.4 27.2 21.2 90.4 48.5 NBY157-MET3-2-MET 60377 44118 2834 580 33.1 38.3 478 95.5 34.8 32.7 NBY157-MET3-4+10xMET 66708 47196 166 104 65.7 51 26 15.6 71.4 44.8 NBY157-MET3-4-MET 57324 41865 1730 530 35.4 43.5 307 91.7 33.4 33.5 NBY157-MET3-5+10xMET 66806 47543 167 111 49.4 50.5 25.9 16.5 52.9 43.6 NBY157-MET3-5-MET 57426 42282 1650 532 35.3 43.9 292 91.7 33.2 33.3 NBY157-MET3-6+10xMET 67015 47187 219 175 49.7 50.2 33.4 25.9 50.3 42.7 NBY157-MET3-6-MET 58792 43729 8395 481 33.3 44.4 1445 81.4 29.3 35.2 NBY157-MET3-7+10xMET 63769 45345 202 140 91.4 51.7 32.8 21.8 102 45.8 NBY157-MET3-7-MET 57738 42457 5708 504 33.1 43.8 1003 86.8 30.5 34.7 NBY157-MET3-9+10xMET 63872 45562 170 105 65.7 48.1 27.8 16.5 69.9 42.2 NBY157-MET3-9-MET 57834 42153 1726 532 37.4 43.4 303 91.2 35.3 33.6 NBY157-TEF1-7 58417 48439 4345 646 35.6 46.1 749 109 33.2 36.3 NBY157-TEF1-8 66224 53026 3030 632 29.7 34.7 461 95.5 30.2 33.4 NBY157-TEF1-9 58434 47610 4382 648 37.6 47.7 751 109 35.4 38.7 NBY195-CYC1-10 78583 53839 237 153 98.8 46.4 30.6 19.5 112 47.6 NBY195-CYC1-12 80196 55907 451 379 45.6 39.2 56.7 47.4 46 37.7 NBY195-CYC1-2 79025 54492 330 219 108 48.5 42.1 27.7 110 47.4 NBY195-CYC1-3 78756 54387 237 148 82.4 43.6 30.4 18.9 82.7 42.8 NBY195-CYC1-4 79946 55181 246 177 47.5 40.2 31.1 22.2 48.5 39.1 NBY195-CYC1-6 77840 53372 249 199 47.6 42.4 32.4 25.6 48.5 41.2 NBY195-CYC1-7 78664 54135 236 154 47.7 40.8 30.3 19.6 48.3 39.7 NBY195-CYC1-8 81007 56349 219 148 86.6 41.8 27.3 18.3 75.9 39.9 NBY195-CYC1-9 79518 54671 241 169 47.6 41.1 30.7 21.3 48.3 39.9 NBY195-LEU1-1+3xLEU 75067 55067 728 484 41.7 38.1 97.6 64.4 41.6 36.3 NBY195-LEU1-10+3xLEU 78818 57091 458 376 55.3 41.4 58.4 47.7 54.2 40 NBY195-LEU1-10-LEU 78449 58243 3597 844 27.3 30.6 461 108 27 29.8 NBY195-LEU1-12+3xLEU 78954 57366 640 438 46 38 81.6 55.5 47.7 37 NBY195-LEU1-12-LEU 79414 59819 5571 776 26.8 30.5 706 98.1 27 30.1 NBY195-LEU1-1-LEU 74322 56377 7138 686 28.3 32.5 966 92.6 27.8 31.6 NBY195-LEU1-2+3xLEU 79121 56566 512 409 45.8 40.2 65 51.7 45.4 38.7 NBY195-LEU1-2-LEU 77797 57563 3767 750 29.4 33.9 487 96.6 29.3 33 NBY195-LEU1-3+3xLEU 77844 56229 478 386 64.4 41.6 61.7 49.6 64.9 40.1 NBY195-LEU1-3-LEU 77502 59574 3640 732 28.6 33.4 472 94.6 28.7 32.5 NBY195-LEU1-5+3xLEU 75249 54598 291 257 59.6 47.6 39 34.2 68.2 46.4 NBY195-LEU1-5-LEU 75443 55482 1880 712 33 35.3 250 94.5 32.6 34.1 NBY195-LEU1-6+3xLEU 77775 55259 327 295 48.3 44.8 42.3 37.8 48.5 43.3 NBY195-LEU1-6-LEU 78656 56680 1944 765 31.9 33.8 248 97.4 32 32.9 NBY195-LEU1-8+3xLEU 79958 57597 320 257 53.3 46.7 40.3 32.2 55.9 44.9 NBY195-LEU1-8-LEU 80234 59321 1975 752 32.8 33.4 247 93.9 32.7 32.7 NBY195-LEU1-9+3xLEU 79104 57415 670 463 45.4 38 85.1 58.6 45.1 36.6 NBY195-LEU1-9-LEU 77464 58468 5455 721 29.1 32.2 707 93.4 28.4 31.7 NBY195-MET17-12+10xMET 78588 53804 369 252 54.8 50.9 47.3 32.1 55 50 NBY195-MET17-12-MET 65681 49829 9780 526 28.1 33.4 1501 80.8 27.9 33.6 NBY195-MET17-2+10xMET 80556 54966 223 163 50.3 45.6 28 20.3 50.8 44.8 NBY195-MET17-2-MET 71200 51525 3377 640 28.4 32.5 478 90.3 28.5 31.5 NBY195-MET17-3+10xMET 80570 54775 223 152 49.5 45.9 27.9 18.9 50.1 44.9 NBY195-MET17-3-MET 69720 49747 3327 626 29 33.4 481 90.2 29.2 32.5 NBY195-MET17-4+10xMET 80416 55647 293 218 54 52 36.7 27.2 54.4 51 NBY195-MET17-4-MET 68577 50356 6947 590 27.5 31.8 1020 86.5 27 31.2 NBY195-MET17-5+10xMET 80015 55661 298 215 64.9 53.5 37.6 26.9 63 52.3 NBY195-MET17-5-MET 68481 50032 6956 574 26.7 32.2 1024 84.4 27 32.1 NBY195-MET17-6+10xMET 79714 55611 223 160 51 48.6 28.3 20.1 51.8 47.7 NBY195-MET17-6-MET 71148 51308 3232 622 28.5 32.1 458 87.8 29.1 31.4 NBY195-MET17-7+10xMET 78609 52954 224 154 49.8 45.8 28.8 19.6 50.4 44.8 NBY195-MET17-7-MET 69386 50006 3197 627 28.8 33 464 90.7 29.2 32.2 NBY195-MET17-9+10xMET 77679 52573 314 240 86.7 51.6 40.8 31 91.1 50.8 NBY195-MET17-9-MET 66899 48179 5882 603 26.8 32.1 886 90.6 26.7 31.1 NBY195-MET3-1+10xMET 77521 53613 177 126 44.5 39 23.1 16.3 46.4 38.6 NBY195-MET3-10+10xMET 78703 54099 181 125 44.7 39 23.3 16 46 38.2 NBY195-MET3-10-MET 72850 51935 1495 549 33 34.8 206 75.7 33.1 34.3 NBY195-MET3-11+10xMET 77646 53140 176 127 44.7 38.8 23 16.5 46.6 38.1 NBY195-MET3-11-MET 72288 50422 1461 577 32.2 33.8 204 80.1 32.8 33 NBY195-MET3-12+10xMET 78656 54261 177 131 44.6 39.7 22.8 16.8 46.3 38.9 NBY195-MET3-12-MET 72373 50914 1476 605 35.7 33.6 206 83.9 36.5 32.8 NBY195-MET3-1-MET 71740 49620 1473 599 32.4 33.7 207 83.6 32.8 32.2 NBY195-MET3-3+10xMET 77369 52423 185 126 167 48.8 24.3 16.3 171 48.6 NBY195-MET3-3-MET 71995 49670 1406 576 36.1 35 196 80.2 35.8 33.7 NBY195-MET3-4+10xMET 78689 54246 191 127 94.5 41.1 24.6 16.2 90.3 40 NBY195-MET3-4-MET 74358 53245 1532 564 33.1 35 207 76.2 32.8 34.2 NBY195-MET3-6+10xMET 77203 53085 178 127 45 38.2 23.3 16.5 46.6 37.6 NBY195-MET3-6-MET 72866 50789 1440 581 32.8 34 199 79.9 32.8 32.8 NBY195-MET3-8+10xMET 77459 53236 180 125 98.8 50.8 23.6 16.2 118 56.2 NBY195-MET3-8-MET 72526 50727 1454 570 32.5 33.9 202 78.8 32.8 32.9 NBY195-MET3-9+10xMET 77036 52775 192 124 92.1 41.4 25.3 16.1 108 42.4 NBY195-MET3-9-MET 70336 50252 2721 602 29.2 33 390 85.9 29.6 32.2 NBY195-TDH3-1 74109 55608 5977 817 27.1 31.2 812 111 26.3 30.3 NBY195-TDH3-10 73185 53078 5840 781 27.9 32.3 802 107 27.2 31.2 NBY195-TDH3-11 72402 52996 5791 783 27.3 32.3 805 108 26.9 31.4 NBY195-TDH3-12 72611 53282 5945 779 27.6 32.4 823 108 27.1 31.4 NBY195-TDH3-2 69336 55945 15600 601 32.6 40.3 2254 87.5 29.6 40.4 NBY195-TDH3-5 72662 54246 5971 799 27.2 31.9 827 110 27 31.1 NBY195-TDH3-6 73415 53794 5889 760 27.6 33.1 807 104 27.3 32.5 NBY195-TDH3-7 68148 55956 15602 582 34.3 36.3 2292 86.3 31.2 36.3 NBY195-TDH3-8 69988 54715 15541 579 33.3 37.4 2226 83.4 30.7 37.2 NBY195-TEF1-1 84146 58213 3753 773 29.2 34.5 449 92.2 29.2 33.4 NBY195-TEF1-10 79737 59580 7304 680 28.7 34.5 923 85.8 28.8 34 NBY195-TEF1-11 76839 56930 11507 655 30.5 34.6 1509 85.9 29.9 34.1 NBY195-TEF1-12 80107 57306 7550 724 30.2 35.2 949 90.8 30.3 34.7 NBY195-TEF1-2 79563 54466 3518 710 29.5 33.9 445 89.6 29.3 33.1 NBY195-TEF1-3 84994 57914 7591 794 29.3 33.6 897 93.6 29.6 33.3 NBY195-TEF1-4 82777 56763 3741 760 29.6 35.1 455 92.1 30 34.6 NBY195-TEF1-5 78900 54719 7137 721 28.2 33.6 912 91.9 28.4 32.9 NBY195-TEF1-6 81358 57106 7311 704 30.4 33.4 905 86.9 30.6 32.8 NBY195-TEF1-7 83005 57084 3648 738 31.7 35 442 89.1 32.3 34 NBY195-TEF1-8 80856 57051 7383 702 28.8 34.4 919 87.2 28.9 33.9 NBY195-TEF1-9 80609 55419 3668 774 28.8 34.8 459 96.3 29.2 34 NBY196-CYC1-1 77984 56362 508 118 49.6 38.3 65.4 15.2 49.5 37.1 NBY196-CYC1-10 72696 54296 279 129 76.6 42.2 38.7 17.8 78.1 41.7 NBY196-CYC1-11 73243 54430 293 125 48.9 38.3 40.5 17.1 49.3 37.9 NBY196-CYC1-12 73609 54422 267 126 49 38.4 36.6 17.3 49.6 37.6 NBY196-CYC1-2 74088 55061 291 129 54.2 41.4 39.6 17.6 53.5 40.3 NBY196-CYC1-3 72837 54439 294 129 115 61.6 40.7 17.7 110 58.9 NBY196-CYC1-4 74176 54649 593 131 42.9 37.5 80.5 17.8 43.2 36.5 NBY196-CYC1-5 72171 53797 273 127 48.8 38.8 38.2 17.7 49.9 38.3 NBY196-CYC1-6 71966 53725 271 121 48.6 38.3 38.1 16.9 49 37.5 NBY196-CYC1-7 73001 54721 289 127 48.3 38.9 40.1 17.4 49 38.4 NBY196-CYC1-8 73048 53636 569 126 45.3 39.9 78.4 17.3 45.2 39 NBY196-CYC1-9 72668 54343 394 125 47.4 38.5 54.6 17.4 47.4 37.6 NBY196-LEU1-1+3xLEU 72900 58614 959 122 38.8 44.2 132 16.7 38 42.4 NBY196-LEU1-10+3xLEU 71677 54652 596 121 45.3 44.4 83.5 16.9 44.7 42.9 NBY196-LEU1-10-LEU 76316 55228 3763 758 29 34.5 495 99.4 28.9 33.4 NBY196-LEU1-12+3xLEU 71660 55010 587 117 49.2 45.8 82.3 16.3 50 45.8 NBY196-LEU1-12-LEU 76382 56211 3802 788 28.1 33 501 103 28.4 31.8 NBY196-LEU1-2+3xLEU 72136 56879 600 118 43 42.3 83.5 16.4 42.7 41.4 NBY196-LEU1-2-LEU 74343 55560 3722 734 28.5 33.7 503 98.7 29.1 33 NBY196-LEU1-4+3xLEU 72134 55780 590 121 44.8 42.9 82.2 16.9 44.8 42.5 NBY196-LEU1-4-LEU 75660 55324 3702 753 28.4 33.5 492 99.7 28.8 32.7 NBY196-LEU1-5+3xLEU 71895 56018 365 119 59.2 47.4 51.1 16.7 57.1 45.6 NBY196-LEU1-5-LEU 76659 55281 2000 519 31.3 42.3 262 67.6 30.9 40.9 NBY196-LEU1-6+3xLEU 71348 55993 342 113 47.8 41.8 48.2 15.8 48 40.8 NBY196-LEU1-6-LEU 75734 55355 1969 491 30.4 42.3 261 64.8 30.1 40.7 NBY196-LEU1-7+3xLEU 71756 55766 571 118 49.2 45.5 79.9 16.5 49.2 44.6 NBY196-LEU1-7-LEU 76013 55474 3765 766 28.5 33.7 498 101 28.6 32.9 NBY196-LEU1-8+3xLEU 71574 55545 352 119 57.5 45 49.4 16.6 54.2 42.6 NBY196-LEU1-8-LEU 76777 55280 2011 512 31.2 42.6 263 66.6 30.8 41.1 NBY196-LEU1-9+3xLEU 69135 52586 617 110 45.6 47.2 89.6 16 45.2 46.1 NBY196-LEU1-9-LEU 73226 53110 3635 739 29.2 33.8 499 101 28.5 32.3 NBY196-MET17-1+10xMET 72138 59432 252 141 52.7 37.9 35.4 19.6 53.3 37 NBY196-MET17-10+10xMET 76418 57378 255 132 67.7 42.7 33.7 17.4 70 42 NBY196-MET17-10-MET 73662 52616 3490 656 29.7 36 477 89.1 29.8 34.1 NBY196-MET17-11+10xMET 75583 57056 450 132 56.8 42.1 59.8 17.5 55.9 41.1 NBY196-MET17-11-MET 67415 51682 11526 554 27.7 33.8 1723 82.7 26.8 32.6 NBY196-MET17-1-MET 74766 53179 3409 662 28.9 34.9 459 88.6 28.9 33.2 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