FIGURE 5 

Left panel: comparison of isoform switching and single-point methods for temperature compensation of WT strains. Open circles: globally compensated system obtained through isoform switching; filled circles: system obtained through single-point (local) compensation at the midpoint of the range assuming identical FRQ pathways the system becomes arrhythmic at the ends of the temperature range; broken line: quadratic curve fitted through the data points. Right panel: simulations of period profile evolution. Filled squares: system obtained by modelling randomised tuning of the locally compensated system to a constant period of 22.5 h through isoform switching (for details, see the Supplementary information, section 8); open squares: partially compensated system observed at an intermediate stage in the tuning process, corresponding to balancing of equation (1) at the upper end of the temperature range; open circles: system obtained through randomised tuning of the locally compensated system assuming identical FRQ pathways.