FIGURE 3 

The impact of rescue deletions. (A) Predicted biomass production for the fbaA mutant of E. coli in arabinose minimal medium as a function of the number of rescue deletions when starting with aceA and sucAB. Deleted rescue genes are indicated in the figure. (B) Biomass production of tpiA- and nuoA-deficient mutants in glucose minimal medium as a function of the number of individual rescue deletions. Deleted genes are indicated in the figure. The optimal biomass flux remains unchanged with the addition of rescue deletions. The biomass fluxes are normalized by the wild-type flux G^wt_FBA=0.745 mmol/g DW-h in (A) and 0.908 mmol/g DW-h in (B).