FIGURE 5
FROM:
Transient heterogeneity in extracellular protease production by Bacillus subtilis
Jan-Willem Veening, Oleg A Igoshin, Robyn T Eijlander, Reindert Nijland, Leendert W Hamoen & Oscar P Kuipers
doi:10.1038/msb.2008.18
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Activation of aprE by DegUhy. Strains (A) aprE-gfp/hy and (B) aprE-gfp/hy/
SU were grown in TY medium and induced with IPTG at the mid-exponential growth phase. After 2 h of induction, cells were collected for flow cytometric analyses. At least 12 000 cells were measured for each strain. (C) Mean fluorescence of three independent experiments is plotted for the indicated IPTG concentrations. Error bars indicate the standard error of the mean. Strain aprE-gfp/hy is indicated as circles (
) and aprE-gfp/hy/SU is shown as open triangles (
). (D) The percentage of cells with a fluorescence level higher than channel 530 is depicted as a function of IPTG concentration. This cutoff was chosen arbitrarily to highlight the differences of highly expressing cells between the strains. The average value of three independent experiments is plotted. Error bars indicate the standard error of the mean.