FIGURE 3
FROM:
Transient heterogeneity in extracellular protease production by Bacillus subtilis
Jan-Willem Veening, Oleg A Igoshin, Robyn T Eijlander, Reindert Nijland, Leendert W Hamoen & Oscar P Kuipers
doi:10.1038/msb.2008.18
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Heterogeneous expression of degU. Strains (A) A:degS-gfp and (B) A:degSU-gfp were grown in TY medium and cells were collected for phase-contrast and fluorescence microscopy. Time is given in hours relative to the transition point between exponential and stationary growth phases (T0). Images are an overlay of both channels. Arrows indicate heterogeneously expressing cells. (C) Quantification of fluorescence microscopy images. Micrographs were quantified and plotted as histograms. Every bin represents a certain percentage of the total population with an average fluorescence level in arbitrary units (AU) within that bin (N=cell count).