FIGURE 2 

Single and double gene deletion predictions. The results of in silico gene deletion predictions are summarized. (A) Single gene deletion predictions are classified into four categories with respect to wild type: lethal (0%), growth reducing (>0% and <90%), growth reducing (>90% and <100%) and no effect (100%). For each case, the percentage of growth is indicated in the parentheses. (B) The lethal single gene deletions grouped according to metabolic processes. (C) The total number of lethal single and double gene deletions. Lethal double gene deletions can be classified as trivial lethal or non-trivial lethal. Trivial lethal implies that at least one of the genes involved is lethal in a single gene deletion. In non-trivial lethal double deletions, both genes involved are not lethal individually as single gene deletions but are lethal in combination.